Nanopore sensing systems

A nanopore sensing system, comprising:

• an application specific integrated circuit (ASIC) sensor mounted on a printed circuit board having an electrical interface with the ASIC sensor;
• and
• a nanopore sequencer formed on the ASIC sensor, the nanopore sequencer including:
• a cis well;
• a cis electrode positioned in the cis well;
• a microporous polymer membrane positioned on the cis electrode, the microporous polymer membrane to confine a redox mediator species therein and to allow an ionic species to pass between the cis electrode and the cis well;
• a plurality of trans wells, each including a trans electrode positioned therein;
• and
• a plurality of nanopores respectively fluidically connecting the cis well to each of the plurality of trans wells.

A nanopore sensing system, comprising:

• an application specific integrated circuit (ASIC) sensor mounted on a printed circuit board having an electrical interface with the ASIC sensor;
• and
• a nanopore sequencer formed on the ASIC sensor, the nanopore sequencer including:
• a cis well;
• a cis electrode positioned in the cis well;
• a non-water soluble and redox active polymer positioned on the cis electrode, wherein reduction or oxidation of the non-water soluble and redox active polymer drives ion diffusion;
• a plurality of trans wells, each including a trans electrode positioned therein;
• and
• a plurality of nanopores respectively fluidically connecting the cis well to each of the plurality of trans wells.