Electrocatalytic polymer device for biological detection
AssigneeOregon State University
InventorsLi-Jing Cheng, Sanjida Yeasmin
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Abstract
Disclosed herein is an electrochemical biosensor comprising at least one working electrode on an electrically insulative substrate. At least one working electrode incorporates an electrocatalytic film, comprising a molecularly imprinted polymer (MIP) layer. In at least one embodiment, MIP layer comprises a plurality of shape-selective cavities and a plurality of non-biological electrocatalytic centers.
Claims26
An electrochemical biosensor, comprising:
- at least one working electrode on an electrically insulative substrate;
- and
- an electrocatalytic film on a surface of the at least one working electrode, wherein the electrocatalytic film comprises a molecularly imprinted polymer (MIP) layer, wherein the MIP layer comprises a plurality of shape-selective cavities, and wherein the electrocatalytic film comprises a plurality of electrocatalytic centers.
A sensor system, comprising:
- an electrochemical biosensor comprising an insulative substrate, wherein at least one working electrode, a reference electrode and a counter electrode are integrated on the insulative substrate, wherein an electrocatalytic film is on the at least one working electrode, wherein the electrocatalytic film comprises a molecular imprinted polymer (MIP) layer, and wherein the electrocatalytic film comprises a non-biological electrocatalyst immobilized within the electrocatalytic film;
- and
- a reader comprising an interface to be coupled to the electrochemical biosensor.
A method for making an electrochemical biosensor assembly, the method comprising:
- patterning an integrated electrochemical cell on an electrically insulative substrate, wherein the integrated electrochemical cell comprises at least one working electrode, a reference electrode and a counter electrode;
- forming an electrocatalytic film on a surface of the at least one working electrode, wherein the electrocatalytic film comprises a plurality of electrocatalytic centers comprising a non-biological electrocatalyst, and a molecular template substance;
- and
- forming shape-selective cavities within the electrocatalytic film, wherein the molecular template substance is removed from the electrocatalytic film.
A method for using an electrochemical biosensor, comprising:
- contacting the electrochemical biosensor with a sample solution comprising a target analyte, wherein the electrochemical biosensor comprises an electrically insulative substrate, wherein an electrochemical cell is integrated on the electrically insulative substrate, wherein the electrochemical cell comprises at least one working electrode, and wherein a molecular imprinted polymer (MIP) layer is on an a surface of the at least one working electrode, and wherein the target analyte selectively binds to a plurality of shape-selective cavities within the MIP layer;
- imposing an electrochemical potential on the at least one working electrode;
- and
- measuring an electrochemical current, wherein the electrochemical current is calibrated to a concentration of the target analyte in the sample solution.
Dependencies are inferred from the claim text.