Methods and Systems for Removing a Histological Stain From a Sample
AssigneeUltivue, Inc.
InventorsKevin Hwang, Grace Elizabeth Vezeau, Edyta Krzymanska-Olejnik
Life of this patent
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Abstract
The present disclosure relates to methods and compositions of removing a histological stain from a histologically stained sample. The method may include contacting the sample with an acidic agent, contacting the sample with a reducing agent, thereby removing the histological stain from the histologically stained sample.
Claims27
A method of removing a histological stain from a histologically stained sample, comprising:
- (a) contacting the sample with an acidic agent, wherein the acidic agent has a pH from 1.0 to 4.0;
- and
- (b) contacting the sample with a reducing agent, wherein the reducing agent has a pH from 8.0 to 10.0,
- thereby removing the histological stain from the histologically stained sample.
A method of preparing a histologically stained sample for a subsequent analysis method to detect at least one target, comprising:
- (a) contacting the sample with an acidic agent, wherein the acidic agent has a pH from 1.0 to 4.0;
- (b) contacting the sample with a reducing agent, wherein the reducing agent has a pH from 8.0 to 10.0,
- (c) contacting the sample with a staining agent to stain at least one target in the sample;
- and
- (d) optionally detecting the at least one target in the sample.
A method of removing a histological stain from a histologically stained sample, comprising:
- (a) contacting the sample with an acidic agent, wherein the acidic agent has a pH from 1.0 to 4.0;
- (b) removing the acidic agent;
- and
- (c) contacting the sample with a reducing agent, wherein the reducing agent has a pH from 8.0 to 10.0,
- thereby removing the histological stain from the histologically stained sample.
A method of preparing a histologically stained sample for a subsequent analysis method to detect at least one target, comprising:
- (a) contacting the sample with an acidic agent, wherein the acidic agent has a pH from 1.0 to 4.0;
- (b) removing the acidic agent;
- and
- (c) contacting the sample with a reducing agent, wherein the reducing agent has a pH from 8.0 to 10.0,
- (d) removing the reducing agent;
- (e) contacting the sample with a staining agent to specifically stain at least one target in the sample;
- and
- (f) optionally detecting the at least one target in the sample.
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A kit for removing a histological stain from a tissue sample, comprising
- (a) an acidic agent having pH from 1.0 to 4.0;
- (b) a reducing agent having a pH from 8.0 to 10.0;
- (c) optionally a rinsing agent;
- and
- (d) optionally a staining reagent.
Dependencies are inferred from the claim text.