Methods of genotyping cannabis

A method for constructing a genetic profile for a Cannabis plant, the method comprising testing a deoxyribonucleic acid (DNA) sample from the plant to determine the length of at least two polymorphic target sequences, thereby constructing the genetic profile of the plant, wherein each polymorphic target sequence comprises a simple sequence repeat (SSR) sequence, wherein the at least two polymorphic target sequences comprise two or more of:

• a target sequence amplifiable by a primer pair having the sequences of SEQ ID NO:
• 1 and SEQ ID NO:
• 2;
• a target sequence amplifiable by a primer pair having the sequences of SEQ ID NO:
• 7 and SEQ ID NO:
• 8;
• a target sequence amplifiable by a primer pair having the sequences of SEQ ID NO:
• 17 and SEQ ID NO:
• 18;
• a target sequence amplifiable by a primer pair having the sequences of SEQ ID NO:
• 19 and SEQ ID NO:
• 20;
• and
• a target sequence amplifiable by a primer pair having the sequences of SEQ ID NO:
• 23 and SEQ ID NO:
• 24;
• wherein testing the DNA sample from the plant to determine the length of at least two polymorphic target sequences comprises:
• amplifying the DNA sample in at least one multiplex reaction with a plurality of primers that specifically hybridize to the at least two polymorphic target sequences to generate amplification products corresponding to the target sequences;
• and determining the length of each amplification product of the at least two polymorphic target sequences,
• wherein the at least one multiplex reaction comprises:
• a primer pair having the sequences of SEQ ID NO:
• 1 and SEQ ID NO:
• 2;
• a primer pair having the sequences of SEQ ID NO:
• 7 and SEQ ID NO:
• 8;
• a primer pair having the sequences of SEQ ID NO:
• 17 and SEQ ID NO:
• 18;
• a primer pair having the sequences of SEQ ID NO:
• 19 and SEQ ID NO:
• 20;
• and
• a primer pair having the sequences of SEQ ID NO:
• 23 and SEQ ID NO:
• 24.

A set of primers for constructing a genetic profile for a Cannabis plant, the set comprising a plurality of primer pairs for specifically amplifying at least five polymorphic target sequences, wherein the at least five polymorphic target sequences comprise:

• a target sequence flanked by SEQ ID NO:
• 1 on the 5′ end and SEQ ID NO:
• 101 on the 3′ end;
• a target sequence flanked by SEQ ID NO:
• 7 on the 5′ end and SEQ ID NO:
• 104 on the 3′ end;
• a target sequence flanked by SEQ ID NO:
• 17 on the 5′ end and SEQ ID NO:
• 109 on the 3′ end;
• a target sequence flanked by SEQ ID NO:
• 19 on the 5′ end and SEQ ID NO:
• 110 on the 3′ end;
• and
• a target sequence flanked by SEQ ID NO:
• 23 on the 5′ end and SEQ ID NO:
• 112 on the 3′ end.