Conjugated Polymeric Particle and Method of Making Same

A method of isolating a target polynucleotide, the method comprising:

• contacting a first solution including the target polynucleotide with a probe conjugated substrate, the probe of the probe conjugated substrate being at least partially complementary to the target polynucleotide, the probe conjugated substrate formed by:
• exchanging a counter ion associated with an oligonucleotide with a lipophilic counter ion to form a biomolecule complex;
• dispersing the biomolecule complex in a nonaqueous solvent;
• and
• coupling the biomolecule complex to a polymeric particle in the presence of the nonaqueous solvent to form the oligonucleotide conjugated polymeric particle;
• washing the probe conjugated substrate while the target polynucleotide is coupled to the probe;
• and
• releasing the target polynucleotide in a second solution.