Transferable type i-f crispr-cas genome editing system

A system for changing, adding, and/or deleting one or more genes in a microbial cell, comprising

• (a) a nucleic acid type I-F cas system vector, comprising
• (i) a type I-F cas operon, comprising the cas genes cas1, cas2-3, cas8f, cas5, cas7 and cas6, and a nucleic acid sequence configured to promote transcription of the cas genes in the microbial cell;
• (ii) one or more genes encoding an integrase enzyme;
• (iii) a nucleic acid sequence recognized as an integration site configured to recognize and attach the vector to a target attachment site within the microbial genome;
• (iv) two nucleic acid sequences configured to be Flp recombinase target sites;
• and
• (v) one or more reporter genes and a nucleic acid sequence configured to promote transcription of the reporter gene(s) upon integration into the microbial cell genome;
• wherein the vector is configured for integration into the genome of the microbial cell via attachment at the target attachment site;
• and
• (b) a nucleic acid editing vector comprising one or more CRISPR RNA (crRNA) nucleic acids and repair donors, configured to change, add, and/or delete one or more target genomic sites in the microbial cell through the type I-F CRISPR-Cas system.