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AssigneeCity University of Hong Kong
InventorsDong SUN, Jiayu SUN
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A system for quantitative control of mitochondrial transfer based on droplet microfluidics includes a droplet generation module configured to generate droplets containing isolated mitochondria and a single cell; a droplet observation module configured for observation of the generated droplets under a microscope; and a droplet collection module configured to collect the generated droplets. The number of mitochondria needed to be transferred into recipient cells is an import issue in precise medicine. The development of the presented invention, which can achieve a precise quantity-control on mitochondrial transfer at the single cell level, can help us to determine the mitochondria number needed to make a significant function improvement on the recipient cells before conducting the cell therapy for mtDNA-related diseases.
A system for quantitative control of mitochondrial transfer based on droplet microfluidics, comprising:
The system according to claim 4, wherein the chip is smaller than 8 cm in length.
The system according to claim 8, wherein the droplet generation module comprises a wave-like structure which is configured to focus randomly distributed cells from the inlet to a line. The system according to claim 9, wherein the wave-like structure is configured to improve the single cell encapsulation ratio more than 47%.
A method for quantitative control of mitochondrial transfer based on droplet microfluidics, comprising the steps of:
Dependencies are inferred from the claim text.