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US 2023/0407323 A1Published application
Delivery of genome editing reagents via chimeric plants
AssigneeCalyxt, Inc.
InventorNathaniel Butler
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Abstract
Embodiments of the present disclosure are directed to a method that includes generating a chimeric plant by infecting a cutting of a host plant with Rhizobium rhizogenes carrying an expression construct, and screening new growth from the chimeric plant for gene edits resulting from genomic editing by the expression construct.
Claims15
A method of delivery of genome editing reagents, comprising:
- generating a chimeric plant by infecting a cutting of a host plant with Rhizobium rhizogenes carrying an expression construct;
- and
- screening new growth from the chimeric plant for gene edits resulting from the expression construct.
A method of generating chimeric plants expressing genome editing reagents, comprising:
- preparing a wild-type shoot or seedling cutting from a host plant;
- inoculating the wild-type shoot or seedling cutting with a Rhizobium rhizogenes solution;
- and
- transferring the wild-type shoot or seeding infected with the R. rhizogenes solution to a medium for growth of transgenic hairy roots.
1 more element
A non-naturally occurring plant, generated by a genomic editing technique, wherein the genomic editing technique includes:
- preparing a cutting of a host plant;
- generating a chimeric plant by infecting the cutting of the host plant with an Rhizobium rhizogenes solution including:
- a transcription activator like effector nuclease (TALEN) messenger ribonucleic acid (mRNA) coding sequence;
- and
- a promoter;
- screening growth from the chimeric plant resulting from genomic editing for genomic edits of a target gene or by detection of the TALEN mRNA and/or protein;
- and
- propagating the growth or seed from the chimeric plant with accumulation of the TALEN mRNA and/or protein for isolation of edited events.
5 more elements
Dependencies are inferred from the claim text.