Modified ribonucleic acids and uses thereof
AssigneeHelix Nanotechnologies Inc
InventorsNikhil Dhar, Marianna Keaveney, Kyle Backman, Everett Webster, Nikolai Eroshenko, Justin Quinn
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Abstract
Disclosed herein is a modified ribonucleotide comprising a nucleoside comprising N4-acetylcytidine and/or 5-hydroxymethyluridine, and polyribonucleotides comprising the same. Also provided herein are compositions comprising a polyribonucleotide of the present disclosure and methods of making and using the same.
Claims31
-21. (canceled)
A method comprising:
- administering a polyribonucleotide or a composition comprising the polyribonucleotide to a cell, tissue or subject, wherein the polyribonucleotide comprises a modified ribonucleotide comprising a nucleoside comprising an acetyl group, wherein the nucleoside is N4-acetylcytidine and the modified ribonucleotide has:
- (a) a 5′ monophosphate;
- (b) a 5′ diphosphate;
- or
- (c) a 5′ triphosphate and a structure of:
A method of manufacturing an RNA composition comprising introducing at least one modified ribonucleotide into a polyribonucleotide, wherein the modified ribonucleotide comprising a nucleoside comprising an acetyl group, wherein the nucleoside is N4-acetylcytidine and the modified ribonucleotide has:
- (a) a 5′ monophosphate;
- (b) a 5′ diphosphate:
- or
- (c) a 5′ triphosphate and a structure of:
(canceled)
A method of obtaining a lower level of immunogenicity in a subject who has received a polyribonucleotide comprising a modified ribonucleotide comprising a nucleoside comprising an acetyl group, wherein the nucleoside is N4-acetylcytidine and the modified ribonucleotide has:
- (a) a 5′ monophosphate;
- (b) a 5′ diphosphate;
- or
- (c) a 5′ triphosphate and a structure of:
- as compared with a subject who has received a comparable unmodified polyribonucleotide, the method comprising administering the polyribonucleotide to the subject.
A method comprising:
- administering a polyribonucleotide to a cell, tissue or subject, wherein the polyribonucleotide comprises a modified ribonucleotide comprising a nucleoside comprising a hydroxymethyl group to a cell, tissue or subject, wherein the nucleoside is 5-hydroxymethyluridine and the modified ribonucleotide has:
- (a) a 5′ monophosphate;
- (b) a 5′ diphosphate;
- or
- (c) a 5′ triphosphate and a structure of
Dependencies are inferred from the claim text.