Novel efficacious microrna-30c analogs reduce apolipoprotein b secretion in human liver cells
OwnerMahmood Hussain, Jia Sheng +2inventors
InventorsMahmood Hussain, Jia Sheng, Pradeep Kumar Yadav, Phensinee Haruehanroengra
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Abstract
Provided are potent miR-30c analogs that can be delivered to hepatoma cells without the aid of viral vectors and lipid emulsions. The miR-30c analogs contain an unmodified active sense strand and a modified passenger strand to enhance the stability and uptake of miR-30c by hepatoma cells. The miR-30c analogs are for use in treatment of hyperlipidemias and cardiovascular diseases.
Claims20
A double stranded polynucleotide complex, the double stranded polynucleotide complex comprising a first strand which optionally comprises no nucleotide modifications and a second strand comprising nucleotide modifications forms the double stranded polynucleotide complex with the first strand, wherein the second strand comprising the modifications comprises a sequence selected from the group sequences consisting of:
- SEQ ID
- NO:
- miR30c-C1
- C1
- miR-30c-1-3p
- (pC)•U•gGgAgAgGgUuGuUuAcUc•C
- 1
- miR30c-C2
- C2
- miR-30c-1-3p
- (pC)•UgGgAgAgGgUuGuUuAc•U•c•C
- 2
- miR30c-C3
- C3
- miR-30c-1-3p
- (pc)•U•gGgAgAgGgUuGuUuAc•U•c•C
- 3
- miR30c-C4
- C4
- miR-30c-1-3p
- GgGaGaGgGuUgUuUaCuC(pC)•u
- 4
- miR30c-C5
- C5
- miR-30c-1-3p
- GgGaGaGgGuUgUuUaCu•C•(pC)•u
- 5
- miR30c-C6
- C6
- miR-30c-1-3p
- C•u•GgGaGaGgGuUgUuUaCuC(pC)u
- 6
- wherein upper case letters signify a 2′-deoxy-2′-fluoro (2′-F) ribosugar modification;
- lower case letters signify a 2′-O-methyl (2′-OMe) ribosugar modification;
- pC signifies 2′-a GalNAc clicked cytidine, and the symbol signifies a phosphorothioate linkage.
Dependencies are inferred from the claim text.