Compositions and methods for nucleic acid isolation

The method of any preceding claim, wherein the oligoethylene glycol is or comprises tetraethylene glycol.

The method of any preceding claim, wherein the salt is selected from or comprises an alkali metal halide, or an alkaline earth metal halide;

• optionally wherein the salt is selected from or comprises sodium chloride, potassium chloride, lithium chloride, magnesium chloride, calcium chloride and barium chloride.

The method of any preceding claim, wherein the salt is or comprises sodium chloride.

The method of any preceding claim, wherein the binding solution comprises buffer providing a pH of from about 6 to about 9;

• optionally wherein the buffer provides a pH of about 7 to about 8.

The method of any preceding claim, wherein the sample i) comprises an in vitro transcription reaction.

The method of any preceding claim, wherein the sample i) is provided as a solution.

The method of any preceding claim, wherein the method further comprises:

• d) washing the separated solid support with bound RNA with a wash buffer and then separating the solid support with bound RNA from the residual solution,
• the wash buffer comprising an aqueous C1-C6 alcohol, and/or an aqueous C2-C10 polyol.

The method of any preceding claim, further comprising:

• e) contacting the separated solid support with bound RNA with an elution buffer, under conditions that release the bound RNA into the elution buffer, thereby providing an eluate and eluted solid support;
• and
• f) collecting the eluate.

A kit comprising:

• a) a binding solution comprising aqueous oligoethylene glycol and a salt, wherein the oligoethylene glycol comprises from about 2 to about 70 ethylene glycol units in linear arrangement, and wherein the oligoethylene glycol is present at a concentration of at least about 35% v/v and the salt is present at a concentration of between about 1 M and about 2 M;
• and
• b) a solid support having a hydrophilic surface.

Use of a binding solution to separate RNA from a sample solution to a solid support,

• wherein the binding solution comprises an oligoethylene glycol at a concentration of at least about 40% v/v and a salt present at a concentration of between about 1.2 M and about 2.5 M,
• wherein the oligoethylene glycol comprises from about 2 to about 70 ethylene glycol units in linear arrangement.