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US 2023/0407274 A1Published application
Compositions and methods involving engineered p27
AssigneeThe Regents of the University of California
InventorsSeth RUBIN, Keelan GUILEY
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Abstract
The disclosure provides polypeptides comprising an engineered p27, or a fragment thereof such polypeptides may be used to form trimeric protein complexes with a cyclin-dependent kinase 4 (Cdk4) (or a variant thereof) or Cdk6 (or a variant thereof), and a cyclin D (CycD) or a variant thereof.
Claims21
-24. (canceled)
A trimeric protein complex comprising:
- (i) a polypeptide comprising an engineered p27 or a fragment thereof, wherein the engineered p27 comprises amino acid substitutions at positions Y74, Y88, and Y89, wherein the amino acid substitution at position Y74 is Y74E, Y74D, or Y74R, the amino acid substitution at Y88 is Y88E or Y88D, and the amino acid substitution at Y89 is Y89E or Y89D;
- (ii) a Cdk4 or a variant thereof, or a Cdk6 or a variant thereof;
- and
- (iii) a CycD or a variant thereof,
- wherein the Cdk4 or the variant thereof or the Cdk6 or the variant thereof in the trimeric protein complex is an active kinase.
2 more elements
-51. (canceled)
Dependencies are inferred from the claim text.