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AssigneesTrans Chromosomics, Inc., National University Corporation Tottori University
InventorsMitsuo OSHIMURA, Toshiaki TABATA, Yasuhiro KAZUKI, Narumi UNO
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An object of the present invention is to provide a method for producing a reversibly immortalized cell which can allow a cell into which an immortalizing gene is introduced to proliferate over a long period without damaging the chromosome of the cell, and is capable of removing the immortalizing gene, and to provide a method for obtaining a large amount of a reversibly immortalized cell that can be cloned and has stable quality. The present invention provides a method for producing a reversibly immortalized cell, comprising the steps of: introducing a chromosomally non-integrated RNA virus vector loaded with one or two or more immortalizing gene(s), selected from the group consisting of Bmi-1 gene, TERT gene, and SV40T gene, into a mammalian cell so that the immortalizing gene is expressed in the cell; and culturing the obtained cell for proliferation.
A method for producing a reversibly immortalized cell, comprising the steps of:
An immortalized cell comprising a removable state of a Sendai virus vector loaded with one or more immortalizing gene(s) selected from the group consisting of Bmi-1 gene, TERT gene, and SV40T gene.
A temperature-sensitive Sendai virus vector loaded with one or two immortalizing gene(s) selected from the group consisting of Bmi-1 gene, TERT gene, and SV40T gene.
Dependencies are inferred from the claim text.