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US 2023/0407266 A1Published application
Primary culture method
AssigneesToppan Printing Co., Ltd., Japanese Foundation for Cancer Research
InventorsYuki TAKAHASHI, Shiro KITANO, Ryohei KATAYAMA, Satoshi NAGAYAMA
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Abstract
A primary culture method in which cells contained in a tissue collected from a living body are primary cultured in vitro, in which the cells in the tissue collected from the living body are seeded and cultured on a top surface of a cell structure containing cells constituting a stroma and composed of a single layer or two or more cell layers laminated in the thickness direction.
Claims16
A primary culture method comprising:
- forming a cell structure being composed of a single layer of first cells constituting the stroma or two or more cell layers of the first cells laminated in the thickness direction, which comprises:
- obtaining a mixture by mixing the first cells, a strong polyelectrolyte, and an extracellular matrix component in a cationic buffer solution;
- seeding the mixture in a cell culture vessel;
- and
- obtaining the cell structure in which first cells are laminated in multiple layers in the cell culture vessel after seeding the mixture, and
- seeding second cells on and in direct contact with a top surface of the cell structure, the second cells recovered a tissue collected from a living body.
3 more elements
A primary cultured cell-containing cell structure comprising:
- a stromal cell layer comprising first cells constituting a stroma, a strong polyelectrolyte and an extracellular matrix component, the stromal cell layer comprising a single layer or two or more cell layers laminated in the thickness direction;
- and
- a cell layer formed from second cells recovered from a tissue collected from a living body on a top surface of the stromal cell layer, wherein
- a thickness of the stromal cell layer is 150 μm or more.
1 more element
Dependencies are inferred from the claim text.