Transformed natural killer cell
AssigneePurdue Research Foundation
InventorsSandro Matosevic, Kyle Lupo
Life of this patent
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Abstract
The present application generally relates to natural killer cells (NK) and genetically modified NK cells, methods of transformation, and methods of use in combination with other molecules for targeting cells of interest.
Claims14
A natural killer cell which is derived from a pluripotent stem cell, stability transformed with a synthetic notch receptor protein construct, said construct comprising, nucleic acid domain segments which encode for, reading from 5′ to 3′;
- a signal peptide, TIGIT/CD155 binding protein, notch core proteins, said notch core domain comprising a series of 3 LNR elements, said notch core domain further sequentially linked to an expressible ADAM/TACE element that is linked to a Ga114-VP64 expressible element.
A method for altering target cell function, where said target cell has CD155 expression, said method comprising contacting said target cell with a natural killer cell which is derived from a pluripotent stem cell, stability transformed with a synthetic notch receptor protein construct, said construct comprising, nucleic acid domain segments which encode for, reading from 5′ to 3′;
- a signal peptide, TIGIT binding protein, notch core proteins, said notch core domain comprising a series of 3 LNR elements, said notch core domain further sequentially linked to an expressible ADAM/TACE element that is linked to a Gal14-VP64 expressible element, for sufficient time so as to alter the cell function of said target cell.
A method for modifying NK cell activity comprising transforming a pluripotent stem cell of NK cell progenitor cell with an expression construct encoding for a TIGIT/CD155 binding protein, notch core protein activation component and an expressible Ga14-VP64 element.
Dependencies are inferred from the claim text.