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US 2023/0407234 A1Published application
Cell concentration methods and devices for use in automated bioreactors
AssigneeLonza Walkersville, Inc.
InventorsJoseph O'CONNOR, Erika MCAFEE, Samatha BANDAPALLE, Yaling SHI, Eytan ABRAHAM
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Abstract
The present disclosure provides cassettes for use in automated cell engineering systems that include cell concentration filters for reducing fluid volume of a cell sample during or following automated processing. The disclosure also provides methods of concentrating a cell population, as well as automated cell engineering systems that can utilize the cassettes and carry out the methods.
Claims20
A cassette for use in an automated cell engineering system, comprising:
- (a) a cell culture chamber;
- (b) a pumping system fluidly connected to the cell culture chamber;
- (c) a tangential flow filter fluidly connected to the pumping system, wherein the pumping system provides a retentate flow to the tangential flow filter and wherein a permeate flow of the tangential flow filter is controlled by a flow controller;
- (d) a satellite volume connected to the tangential flow filter;
- (e) a fluidics pathway for recirculating the retentate flow back through the tangential flow filter;
- (f) a fixed volume waste collection chamber fluidly connected to the tangential flow filter;
- and
- (g) a cellular sample output fluidly connected to the tangential flow filter.
5 more elements
An automated cell engineering system, comprising:
- (a) an enclosable housing;
- (b) a cassette contained within the enclosable housing, the cassette comprising:
- i. a cell culture chamber;
- ii. a pumping system fluidly connected to the cell culture chamber;
- iii. a tangential flow filter fluidly connected to the pumping system, wherein the pumping system provides a retentate flow to the tangential flow filter and wherein a permeate flow of the tangential flow filter is controlled by a flow controller;
- and
- iv. a cellular sample output fluidly connected to the tangential flow filter;
- and
- (c) a user interface for receiving input from a user.
6 more elements
Dependencies are inferred from the claim text.