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US 2023/0407222 A1Published application
Temperature calibration methods and devices for use in automated bioreactors
AssigneesOctane Biotech Inc., Lonza Walkersville, Inc.
InventorsPaul GARDIN, Raelyn DANIELS, Talib ALI, Guy ORAM, Matthew HEWITT, Eytan ABRAHAM
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Abstract
The present disclosure provides devices and associated methods for temperature monitoring and control in automated biological material engineering systems, including cell engineering systems. The devices and methods utilize measurement of internal temperatures in an automated system to map temperatures during the various processes carried out in the systems.
Claims39
A calibration cassette for use in an automated biological material engineering system, comprising:
- a low temperature chamber including a media storage vessel and a first array of sealed temperature probes in the media storage vessel;
- a high temperature chamber separated from the low temperature chamber by a thermal barrier, the high temperature chamber including a cell culture chamber and a second array of sealed temperature probes in the cell culture chamber;
- one or more fluidics pathways connected to the cell culture chamber and the media storage vessel, and including a third array of sealed temperature probes in the one or more fluidics pathways;
- and
- electrical connection elements that are electrically connected to each of the first, second, and third arrays of sealed temperature probes.
2 more elements
A production cassette for use in an automated cell engineering system, comprising:
- a low temperature chamber including a cell culture media storage vessel and a first array of sealed temperature probes in the cell culture media storage vessel;
- a high temperature chamber for carrying out activation, transduction and/or expansion of a cell culture, the high temperature chamber separated from the low temperature chamber by a thermal barrier, the high temperature chamber including a cell culture chamber and a second array of sealed temperature probes in the cell culture chamber;
- one or more fluidics pathways connected to the cell culture chamber and the cell culture media storage vessel, and including a third array of sealed temperature probes in the one or more fluidics pathways;
- and
- electric connection elements that are electrically connected to each of the first, second, and third arrays of sealed temperature probes,
- wherein the one or more fluidics pathways provide recirculation, removal of waste, and homogenous gas exchange and distribution of nutrients to the cell culture chamber.
3 more elements
A method of temperature monitoring in an automated biological material engineering system, the method comprising:
- receiving, by a control circuit, a set of internal temperature measurements during a time period when a first cassette is housed in the automated biological material engineering system, wherein the set of internal temperature measurements indicate temperature within the first cassette, and are generated during the time period by an array of temperature probes disposed within the first cassette;
- receiving, by the control circuit, an ambient temperature measurement when the first cassette is housed in the automated biological material engineering system, wherein the ambient temperature measurement indicates temperature outside the first cassette, and is generated during the time period by a system temperature probe of the automated biological material engineering system that is disposed outside the first cassette;
- and
- determining, by the control circuit, a set of temperature offset values that indicate respective differences between the set of internal temperature measurements and the ambient temperature measurement.
1 more element
A method of temperature control performed in automated biological material engineering system, the method comprising:
- receiving, by a control circuit, a set of internal temperature measurements during a first time period when a first cassette is housed in the automated biological material engineering system, wherein the set of internal temperature measurements indicate temperature within the first cassette, and are generated during the first time period by an array of temperature probes disposed within the first cassette;
- receiving, by the control circuit, a first ambient temperature measurement when the first cassette is housed in the automated biological material engineering system, wherein the first ambient temperature measurement indicates temperature outside the first cassette, and is generated during the first time period by a system temperature sensor of the automated biological material engineering system that is disposed outside the first cassette;
- determining, by the control circuit, a set of temperature offset values that indicate respective differences between the set of internal temperature measurements and the first ambient temperature measurement;
- determining, by the control circuit, a target internal temperature value for a location in a second cassette;
- and
- controlling, by the control circuit during a second time period when the second cassette is housed in the automated biological material engineering system, a heating device or cooling device of the automated biological material engineering system based on the target internal temperature value, the set of temperature offset values, and one or more additional ambient temperature measurement generated by the system temperature sensor during the second time period, wherein the system temperature sensor is disposed outside the second cassette.
3 more elements
Dependencies are inferred from the claim text.