Method for evaluating cystine uptake ability of cell, kit for evaluating cystine uptake ability of cell, method for determining selenocysteine, and kit for determining selenocysteine

A kit for evaluating cystine uptake ability of cells, comprising:

• a reducing agent that reduces selenocystine to selenocysteine,
• a fluorescent dye represented by the following general formula (I), and
• a buffer solution at a pH of 5.5 to 6.5,
• wherein A represents an acryloyl group or a methacryloyl group, R2, R3, R4, R5, and R6 are each independently an atom or an atomic group selected from the group consisting of a hydrogen atom, a hydroxyl group, a thiol group, a halogen atom, an amino group, a sulfonamide group, an azido group, and a cyano group;
• and a linear alkyl group, a branched alkyl group, a cycloalkyl group, an aryl group, and a heteroaryl group in which one or more hydrogen atoms are optionally replaced with other atoms or functional groups and that optionally contain one or more of an amino group, a carbonyl group, an oxygen atom, and a sulfur atom in carbon skeletons, and among R2, R3, R4, R5, and R6, any two adjacent ones share atoms, forming a cyclic fluorescent moiety where the fluorescence wavelength and fluorescence intensity change one or both, due to the conversion of the 0-A group to an OH group.

A kit for determining selenocysteine, comprising:

• a fluorescent dye represented by the following general formula (I) and
• a buffer solution at a pH of 5.5 to 6.5,
• wherein A represents an acryloyl group or a methacryloyl group, R2, R3, R4, R5, and R6 are each independently an atom or an atomic group selected from the group consisting of a hydrogen atom, a hydroxyl group, a thiol group, a halogen atom, an amino group, a sulfonamide group, an azido group, and a cyano group;
• and a linear alkyl group, a branched alkyl group, a cycloalkyl group, an aryl group, and a heteroaryl group in which one or more hydrogen atoms are optionally replaced with other atoms or functional groups and that optionally contain one or more of an amino group, a carbonyl group, an oxygen atom, and a sulfur atom in the carbon skeletons, and among R2, R3, R4, R5, and R6, any two adjacent ones share atoms, forming a cyclic fluorescent moiety where the fluorescence wavelength and fluorescence intensity change one or both, due to the conversion of the 0-A group to an OH group.