Immunogenic compositions comprising conjugated capsular saccharide antigens and uses thereof

A method of detecting the presence of:

• 4-keto-N-acetyl-quinovosamine residues in an isolated S. pneumoniae serotype 12F polysaccharide, said method comprising the step of:
• a) isolating an S. pneumoniae serotype 12F polysaccharide and b) detecting the presence of 4-keto-N-acetyl-quinovosamine residues in said polysaccharide;
• N-acetyl-D-fucosamine (D-FucNAc) residues in a reduced serotype 12F polysaccharide, said method comprising the step of:
• a) reacting an isolated S. pneumoniae serotype 12F polysaccharide with a reducing agent and b) detecting the presence of N-acetyl-D-fucosamine (D-FucNAc) residues in said reduced polysaccharide;
• N-acetyl-D-quinovosamine (D-QuiNAc) residues in a reduced serotype 12F polysaccharide, said method comprising the step of:
• a) reacting an isolated S. pneumoniae serotype 12F polysaccharide with a reducing agent and b) detecting the presence of N-acetyl-D-quinovosamine (D-QuiNAc) residues in said reduced polysaccharide;
• N-acetyl-D-fucosamine (D-FucNAc) and N-acetyl-D-quinovosamine (D-QuiNAc) residues in a reduced serotype 12F polysaccharide, said method comprising the step of:
• a) reacting an isolated S. pneumoniae serotype 12F polysaccharide with a reducing agent and b) detecting the presence of N-acetyl-D-fucosamine (D-FucNAc) and N-acetyl-D-quinovosamine (D-QuiNAc) residues in said reduced polysaccharide;
• N-acetyl-D-fucosamine (D-FucNAc) and/or N-acetyl-D-quinovosamine (D-QuiNAc) residues in S. pneumoniae serotype 12F glycoconjugate, said method comprising the step of:
• a) preparing a S. pneumoniae serotype 12F glycoconjugate and b) detecting the presence of N-acetyl-D-fucosamine (D-FucNAc) and/or N-acetyl-D-quinovosamine (D-QuiNAc) residues in said glycoconjugate;
• N-acetyl-D-fucosamine (D-FucNAc) residues in S. pneumoniae serotype 12F glycoconjugate, said method comprising the step of:
• a) preparing a S. pneumoniae serotype 12F glycoconjugate and b) detecting the presence of N-acetyl-D-fucosamine (D-FucNAc) residues in said glycoconjugate;
• and/or
• N-acetyl-D-quinovosamine (D-QuiNAc) residues in S. pneumoniae serotype 12F glycoconjugate, said method comprising the step of:
• a) preparing a S. pneumoniae serotype 12F glycoconjugate and b) detecting the presence of N-acetyl-D-quinovosamine (D-QuiNAc) residues in said glycoconjugate.