System and method for cold atmospheric plasma based t cell therapy

Abstract

A method for using Cold Atmospheric Plasma based T cell therapy for treating cancer including treating tumor cells with cold atmospheric plasma to permeabilize the tumor cells, condensing a mammalian CRISPR plasmid vector into compact nanostructures using one of poly-L-lysine (PLL) and Star-shaped poly(ethylene glycol)-block-polyethylenimine, and transfecting tumor cells with the catalytically inactive condensed CRISPR plasmid vector containing transcriptional activators (dcas9-VP64-sgRNA-CRISPRa) to elicit immune responses by enhancing the presentation of tumor-associated antigens.

Dependencies are inferred from the claim text.