Diagnostic
AssigneeHAV Vaccines Limited
InventorJohn HERMON-TAYLOR
Life of this patent
Estimating the term…
Abstract
A method of diagnosing or monitoring Mycobacterium avium subspecies paratuberculosis (MAP) infection, which method comprises detecting the presence of the polypeptide (MAP P900) encoded by the positive strand of IS900, or a fragment thereof, in a sample from a subject, wherein MAP P900, or a fragment thereof, is detected using an antibody, or an antigen-binding fragment thereof, that binds to MAP P900.
Claims22
-36. (canceled)
A method of detecting Mycobacterium avium subspecies paratuberculosis (MAP), wherein the method comprises detecting the presence of the polypeptide (MAP P900) encoded by the positive strand of IS900, or a fragment thereof, by detecting an extracellular region of MAP P900, wherein the extracellular region of MAP P900 is selected from the group consisting of:
- MVINDDAQRLLSQR (SEQ ID NO:
- 2), MVINDDAQRLL[pS]QR (SEQ ID NO:
- 3), YLSALVSIRTDPSSR (SEQ ID NO:
- 4), VSIRTDPSSR (SEQ ID NO:
- 5), YLSALVSIRTDPS[pS]R (SEQ ID NO:
- 6), YLSALVSIRTDP[pS]SR (SEQ ID NO:
- 7), VSIRTDP[pS][pS]R (SEQ ID NO:
- 8), VSIRTDP[pS]SR (SEQ ID NO:
- 9), AAVTTLADGGEVTWAID (SEQ ID NO:
- 10), NLKRPRRYDRRLLRA (SEQ ID NO. 11), VTTLADGGEVTWAID (SEQ ID NO:
- 12), YLSALVSIRTDP[pS][pS]R (SEQ ID NO:
- 13) and VSIRTDPS[pS]R (SEQ ID NO:
- 98).
A method of food safety testing, wherein the method comprises detecting MAP in a food sample, wherein the detection of MAP comprises detecting the presence of MAP P900, or a fragment thereof, by detecting an extracellular region of MAP P900 in the food sample, wherein the extracellular region of MAP P900 is selected from the group consisting of:
- MVINDDAQRLLSQR (SEQ ID NO:
- 2), MVINDDAQRLL[pS]QR (SEQ ID NO:
- 3), YLSALVSIRTDPSSR (SEQ ID NO:
- 4), VSIRTDPSSR (SEQ ID NO:
- 5), YLSALVSIRTDPS[pS]R (SEQ ID NO:
- 6), YLSALVSIRTDP[pS]SR (SEQ ID NO:
- 7), VSIRTDP[pS][pS]R (SEQ ID NO:
- 8), VSIRTDP[pS]SR (SEQ ID NO:
- 9), AAVTTLADGGEVTWAID (SEQ ID NO:
- 10), NLKRPRRYDRRLLRA (SEQ ID NO:
- 11), VTTLADGGEVTWAID (SEQ ID NO:
- 12), YLSALVSIRTDP[pS][pS]R (SEQ ID NO:
- 13) and VSIRTDPS[pS]R (SEQ ID NO:
- 98).
A method of diagnosing and treating MAP infection in a human or animal subject, wherein the method comprises:
- (a) detecting MAP, wherein the detection of MAP comprises detecting the presence of MAP P900, or a fragment thereof, in a sample from the subject, by detecting an extracellular region of MAP P900 in the sample, wherein the extracellular region of MAP P900 is selected from the group consisting of:
- MVINDDAQRLLSQR (SEQ ID NO:
- 2), MVINDDAQRLL[pS]QR (SEQ ID NO:
- 3), YLSALVSIRTDPSSR (SEQ ID NO:
- 4), VSIRTDPSSR (SEQ ID NO:
- 5), YLSALVSIRTDPS[pS]R (SEQ ID NO:
- 6), YLSALVSIRTDP[pS]SR (SEQ ID NO:
- 7), VSIRTDP[pS][pS]R (SEQ ID NO:
- 8), VSIRTDP[pS]SR (SEQ ID NO:
- 9), AAVTTLADGGEVTWAID (SEQ ID NO:
- 10), NLKRPRRYDRRLLRA (SEQ ID NO:
- 11), VTTLADGGEVTWAID (SEQ ID NO:
- 12), YLSALVSIRTDP[pS][pS]R (SEQ ID NO:
- 13) and VSIRTDPS[pS]R (SEQ ID NO:
- 98);
- and
- (b) treating the human or animal subject in whom MAP is detected, optionally wherein the human or animal subject is treated with an antimicrobial agent, a therapeutic MAP vaccine or passive immunotherapy.
A peptide fragment of MAP P900 of up to 40 amino acids comprising one or more of the following sequences, MVINDDAQRLLSQR (SEQ ID NO:
- 2), MVINDDAQRLL[pS]QR (SEQ ID NO:
- 3), YLSALVSIRTDPSSR (SEQ ID NO:
- 4), VSIRTDPSSR (SEQ ID NO:
- 5), YLSALVSIRTDPS[pS]R (SEQ ID NO:
- 6), YLSALVSIRTDP[pS]SR (SEQ ID NO:
- 7), VSIRTDP[pS][pS]R (SEQ ID NO:
- 8), VSIRTDP[pS]SR (SEQ ID NO:
- 9), AAVTTLADGGEVTWAID (SEQ ID NO:
- 10), NLKRPRRYDRRLLRA (SEQ ID NO:
- 11), VTTLADGGEVTWAID (SEQ ID NO:
- 12), YLSALVSIRTDP[pS][pS]R (SEQ ID NO:
- 13) and VSIRTDPS[pS]R (SEQ ID NO:
- 98).
Dependencies are inferred from the claim text.