Device and method for making cell sheets
AssigneeCaroMeats Inc.
InventorAlireza Shahin-Shamsabadi
Life of this patent
Estimating the term…
Abstract
Systems and methods for growing cells or cell sheets are described. The method may use biocompatible or food-safe materials. The method may allow for cell alignment, optionally in selected patterns, which may be produced by casting a membrane on a 3D printed mold. The method may include surface treatment of an elastomeric membrane. The method may allow for the reuse of the membranes by autoclaving and/or a washing step. The method may create multi-layer cell sheets with an extracellular matrix (ECM) created by the cells, which may be detached from the membrane. Optionally, the cells may be separated from the ECM.
Claims20
A substrate for growing cells or a cell sheet comprising,
- an elastomeric membrane having a patterned surface;
- and,
- a hydrophilic agent attached to the surface of the elastomeric membrane.
A method of making a substrate for growing cells or a cell sheet, the method comprising,
- casting an elastomeric membrane on a mold having a patterned surface;
- and,
- attaching a hydrophilic agent to the elastomeric membrane.
A method of creating a cell construct, the method comprising:
- providing an elastomeric membrane with a hydrophilic and patterned surface;
- growing cells on the membrane in layers, wherein the cells produce an extra-cellular matrix (ECM);
- and,
- removing the layers from the membrane.
The method of any of claims 91 to 93 comprising producing the patterned surface by casting the membrane on a patterned mold.
The method of any of claims 91 to 95 wherein the membrane comprises a hydrophilic agent, wherein a) the hydrophilic agent is not an exogenous extra-cellular matrix (ECM) component or b) the hydrophilic agent comprises a polyphenol substantially without iron.
Dependencies are inferred from the claim text.