Cell compositions comprising antigen-specific t cells for adoptive therapy

An isolated cell composition suitable for adoptive immunotherapy, the composition comprising, in a pharmaceutically acceptable carrier:

• at least 90% CD8+ T cells and less than 5% CD4+ T cells;
• the CD8+ cells comprising at least 106 CD8+ T cells specific for from 1 to 10 target peptide antigens, and CD8+ T cells specific for bacterial, viral, fungal and/or parasitic pathogens,
• wherein at least 30% of the CD8+ T cells are central memory and effector memory T cells with a ratio of from 25:75 to 75:25, with less than 10% of the CD8+ T cells being terminally differentiated T cells and less than 10% of the CD8+ cells being naive cells;
• and
• wherein at least 50% of the CD8+ T cells specific for the target peptide antigens are central memory and effector memory T cells with a ratio of from 25:75 to 75:25, are less than 10% terminally differentiated T cells, and are less than 10% naive cells.