Bead-Enabled, Efficient, and Rapid Multi-Omic Sample Preparation for Mass Spectrometry Analysis

A method for extracting biomolecules from a sample comprising the steps of:

• a) mixing the sample with an extraction solvent and a plurality of unmodified immobilizing beads, wherein the extraction solvent comprises, by volume, between 20% and 80% of n-butanol and is able to solubilize a first portion of biomolecules comprising lipids and metabolites, and wherein the plurality of unmodified immobilizing beads are able to bind and immobilize a second portion of biomolecules comprising proteins and polypeptides,
• thereby generating a monophasic extraction solution comprising the first portion of biomolecules and generating bound immobilizing beads attached to the second portion of biomolecules;
• b) separating the bound immobilizing beads attached to the second portion of biomolecules from the extraction solution comprising the first portion of biomolecules;
• c) mixing the bound immobilizing beads attached to the proteins and polypeptides with a protein digestion enzyme or chemical agent for a digestion time period between 35 minutes and 45 minutes at a digestion temperature between 55° C. and 65° C.;
• and
• d) separating the first portion of biomolecules from the extraction solution, thereby generating at least a first set of extracted biomolecules, and separating the second portion of biomolecules from the bound immobilizing beads, thereby generating at least a second set of extracted biomolecules,
• wherein steps a) through d) are performed within three hours or less.