Engineered biosynthetic pathway for production of 4-aminophenylethylamine by fermentation
AssigneeZymergen Inc.
InventorsZhangying Hao, Shuchi Desai, Steven M. Edgar, Joseph Gallagher, James Chandler, Eyal Akiva, Rebecca Davidson, Matthew Davis
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Abstract
The present disclosure describes the engineering of microbial cells for fermentative production of 4-APEA and related products and provides novel engineered microbial cells and cultures, as well as related 4-APEA production methods.
Claims24
An engineered microbial cell that produces 4-aminophenylethylamine (4-APEA), wherein the engineered microbial cell has a high tolerance to toxicity associated with the production of 4-APEA, as defined by a concentration at which the growth of engineered microbial cell is slowed by half (Ki) of at least 30 grams/liter, wherein the engineered microbial cell optionally comprises a yeast cell, optionally a cell of the genus Komagataella, optionally wherein the yeast cell is a cell of the species pastoris or phaffi.
An engineered microbial cell of the genus Komagataella that produces 4-aminophenylpyruvate (4-APP), optionally wherein the engineered microbial cell is a cell of the species pastoris or phaffi.
Dependencies are inferred from the claim text.