Extracorporeal device and matrix for removing ammonia from biological fluids, methods and uses thereof

A device comprising:

• a housing having at least one fluid inlet port, and at least one fluid outlet port;
• the housing including at least one chamber, said at least one chamber defining a control volume in fluid communication with the at least one fluid inlet port and the at least one fluid outlet port;
• said control volume accommodating at least one of a conjugate, a plurality of conjugates or at least one composition comprising said conjugates or plurality of conjugates, said conjugate having the structure of formula I, the conjugate comprising a particle bonded to at least one linker comprising a chain of n carbon atoms covalently bonded to m carbonyl groups, and at least one trapping agent A covalently bonded to the mth carbonyl group;
• wherein, n is an integer within the range of 5 to 15, and m is an integer within the range of 5 to 10, wherein trapping agent A is characterized by having the ability to capture or bind amine;
• optionally, said amine is at least one of methylamine, dimethylamine or trimethylamine.

An extracorporeal apparatus comprising at least one conjugate, or at least one device comprising said conjugate, or connected to said at least one device or battery of devices, wherein said conjugate comprising a particle bonded to at least one linker comprising a chain of n carbon atoms covalently bonded to m carbonyl groups, and at least one trapping agent A covalently bonded to the mth carbonyl group,

• wherein, n is an integer within the range of 5 to 15, and m is an integer within the range of 5 to 10, wherein trapping agent A is characterized by having the ability to capture or bind amine;
• optionally, said amine is at least one of methylamine, dimethylamine or trimethylamine, and wherein said device comprising:
• a housing having at least one fluid inlet port, and at least one fluid outlet port;
• the housing including at least one chamber, said at least one chamber defining a control volume in fluid communication with the at least one fluid inlet port and the at least one fluid outlet port;
• said control volume accommodating said at least one of a conjugate, a plurality of conjugates or at least one composition comprising said conjugates or plurality of conjugates, optionally, said extracorporeal apparatus is for use in depleting ammonia from mammalian body fluid/s.

A method for depleting at least one amine from body fluid/s of a subject in need thereof by an extracorporeal procedure, the method comprising the steps of:

• (i) transferring body fluids of said subject into an extracorporeal apparatus;
• (ii) subjecting said body fluid to affinity depletion procedure specific for at least one amine, wherein said depletion is performed before, during or after blood is being transferred into and out-off said apparatus, thereby obtaining an extracorporeal body fluid of said subject depleted in at least one amine;
• and
• (iii) reintroducing or returning said body fluid obtained in step (ii) to said subject;
• wherein said affinity-depletion procedure comprises contacting said body fluid with an effective amount of conjugates, a plurality of conjugates or a composition thereof comprised within said extracorporeal apparatus, or within a device or battery connected to said extracorporeal apparatus, wherein each conjugate comprises a particle bonded to at least one linker comprising a chain of n carbon atoms covalently bonded to m carbonyl groups, and at least one trapping agent A covalently bonded to the mth carbonyl group,
• wherein, n is an integer within the range of 5 to 15, and m is an integer within the range of 5 to 10, wherein trapping agent A is characterized by having the ability to capture or bind amine;
• optionally, said amine is at least one of methylamine, dimethylamine or trimethylamine, optionally, wherein said conjugate is having the structural formula V, the conjugate comprising a particle covalently bonded to at least one linker comprising a chain of 15 carbon atoms covalently bonded to m carbonyl groups, and a sulfonic acid covalently bonded to the mth carbonyl group
• wherein m is an integer between 5 to 10.