Probiotic bacillus compositions and methods of use

A method of preparing a fermentation product comprising the steps of:

• (a) obtaining at least one bacterial strain selected from a first isolated Bacillus amyloliquefaciens strain comprising SEQ ID NO:
• 5 or comprising SEQ ID NO:
• 261 or nucleic acid encoding one or more of SEQ ID NOs:
• 263-276, a second isolated Bacillus amyloliquefaciens strain comprising SEQ ID NO:
• 10 or 11 or comprising SEQ ID NO:262 or nucleic acid encoding one or more of SEQ ID NOs:
• 277-284, and a first isolated Bacillus subtilis strain comprising SEQ ID NO:
• 257 or comprising nucleic acid encoding one or more of SEQ ID NOs:
• 285-305;
• (b) contacting the at least one strain of step (a) with cell growth media;
• (c) incubating a combination of at least one strain of step (a) and cell growth media of step (b) at a temperature of about 37° C. for an incubation time of about 24 hours;
• and
• (d) cooling the combination of step (c);
• wherein the product of step (d) comprises the fermentation product.

A method of delivering a metabolite to the gut of an animal, said method comprising administering to an animal a composition comprising:

• a first isolated Bacillus amyloliquefaciens strain comprising SEQ ID NO:
• 5 or comprising SEQ ID NO:
• 261 or nucleic acid encoding one or more of SEQ ID NOs:
• 263-276, and a second isolated Bacillus amyloliquefaciens strain comprising SEQ ID NO:
• 10 or 11 or comprising SEQ ID NO:262 or nucleic acid encoding one or more of SEQ ID NOs:
• 277-284;
• wherein the metabolite comprises at least one of:
• histidine, N-acetylhistidine, phenyllactate (PLA), 1-carboxyethyltyrosine, 3-(4-hydroxyphenyl)lactate (HPLA), tryptophan, N-acetyltryptophan, anthranilate, indolelactate, isovalerylglycine, N-acetylisoleucine, N-acetylmethionine, urea, ornithine, spermidine, spermine, cysteinylglycine, pyruvate, sucrose, fumarate, deoxycarnitine, 2R,3R-dihydroxybutyrate, chiro-inositol, glycerophosphorylcholine (GPC), 5-aminoimidazole-4-carboxamide, xanthine, AMP, 2′-deoxyadenosine, dihydroorotate, UMP, uridine, CMP, cytidine, (3′-5′)-adenylyluridine, (3′-5′)-cytidylyladenosine, (3′-5′)-cytidylylcytidine, (3′-5′)-cytidylyluridine, (3′-5′)-guanylylcytidine, (3′-5′)-guanylyluridine, (3′-5′)-uridylylcytidine, (3′-5′)-uridylyluridine, (3′-5′)-uridylyladenosine, NAD+, oxalate (ethanedioate), maltol, 1-methylhistidine, N6,N6-dimethyllysine, S-methylcysteine, and 2-methylcitrate.