Urine-derived epithelial cell cultures, nephrospheroids derived therefrom and methods of producing and using same
OwnerBenjamin DEKEL, Dorit OMER +1inventors
InventorsBenjamin DEKEL, Dorit OMER, Orit HARARI-STEINBERG
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Abstract
A nephrospheroid comprising urine-derived epithelial cells, the nephrospheroid is capable of forming a tubular nephric tissue upon transplantation. Also provided are methods of producing the nephrospheroid and using same.
Claims38
A method of expanding kidney epithelial cells, the method comprising:
- (a) isolating cells from urine of a subject;
- (b) culturing said cells under adherent conditions, so as to obtain urine-derived epithelial cells (UD-EpC);
- (c) passaging said UD-EpC.
A nephrospheroid comprising urine-derived epithelial cells, the nephrospheroid is capable of forming a tubular nephric tissue upon transplantation and/or having an anti-fibrotic activity.
A method of analyzing infectivity of a Coronavirus, the method comprising:
- (a) contacting a renal epithelial cell culture or a nephrospheroid produced of said culture with a Coronavirus;
- and
- (b) determining infectivity of said Coronavirus in the culture or in the nephrospheroid following said contacting.
A method of personalized therapy, the method comprising:
- (a) contacting a Coronavirus-infected renal epithelial cell culture or nephrospheroid produced of said culture with a test drug;
- and
- (b) determining an alleviation in viral load following said contacting, said alleviation being indicative of an efficacious therapy.
Dependencies are inferred from the claim text.