Modular dendron micelles for combination immunotherapy
AssigneeWisconsin Alumni Research Foundation
InventorsSeungpyo Hong, Deric L. Wheeler
Life of this patent
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Abstract
A self-assembled immunotherapeutic dendron-micelle includes a first amphiphilic dendron-coil, a second amphiphilic dendron-coil, and a third amphiphilic dendron-coil. The first and second amphiphilic dendron-coils have immunotherapeutic peptides conjugated thereto. Also included are pharmaceutical compositions containing the dendron-micelles, methods of making the dendron-micelles, and immunotherapy methods including administering the dendron-micelles to a subject in need thereof.
Claims19
A self-assembled immunotherapeutic dendron-micelle, comprising
- a first amphiphilic dendron-coil, a second amphiphilic dendron-coil, and a third amphiphilic dendron-coil;
- wherein the first amphiphilic dendron-coil comprises a first non-peptidyl, hydrophobic core-forming component covalently linked to a first polyester dendron which is covalently linked to first a poly(ethylene glycol) (PEG) moiety, wherein the first PEG moiety comprises a first conjugated immunotherapeutic peptide;
- wherein the second amphiphilic dendron-coil comprises a second non-peptidyl, hydrophobic core-forming component covalently linked to a second polyester dendron which is covalently linked to a second poly(ethylene glycol) (PEG) moiety, wherein the second PEG moiety comprises a second conjugated immunotherapeutic peptide;
- and
- wherein the third amphiphilic dendron-coil comprises a third non-peptidyl, hydrophobic core-forming component covalently linked to a third polyester dendron which is covalently linked to a third poly(ethylene glycol) (PEG) moiety, wherein the third PEG moiety does not comprise a conjugated immunotherapeutic peptide.
A method of making a self-assembled immunotherapeutic dendron-micelle, comprising
- synthesizing a first amphiphilic dendron-coil by covalently linking a first non-peptidyl, hydrophobic core-forming component to a first polyester dendron, covalently linking the first polyester dendron to a first poly(ethylene glycol) (PEG) moiety, and conjugating a first therapeutic peptide to the first PEG moiety;
- synthesizing a second amphiphilic dendron-coil by covalently linking a second non-peptidyl, hydrophobic core-forming component to a second polyester dendron, covalently linking the second polyester dendron to a second poly(ethylene glycol) (PEG) moiety, and conjugating a second therapeutic peptide to the second PEG moiety;
- synthesizing a third amphiphilic dendron-coil by covalently linking a third non-peptidyl, hydrophobic core-forming component to a third polyester dendron, covalently linking the third polyester dendron to a third poly(ethylene glycol) (PEG) moiety, wherein the third PEG moiety does not comprise a conjugated immunotherapeutic peptide;
- and
- incubating the first, second, and optionally third amphiphilic dendron-coils under conditions for self-assembly of the self-assembled immunotherapeutic dendron-micelle.
Dependencies are inferred from the claim text.