Variant of nitrous oxide reductase protein and method of reducing concentration of nitrous oxide in sample
AssigneesSamsung Electronics Co., Ltd., Konkuk University Industrial Cooperation Corp.
InventorsWoo Yong Shim, Seung Hoon Song, Anurag Kumar, Jung-Kul Lee, Jae-Young Kim, Yu Kyung Jung
Life of this patent
Estimating the term…
Abstract
Provided are polynucleotides encoding variants of nitrous oxide reductase proteins, recombinant microorganisms including a foreign gene encoding a variant of a nitrous oxide reductase protein, and a composition comprising the recombinant microorganism or the variant of a nitrous oxide reductase protein for use in removing nitrous oxide in a sample.
Claims20
A recombinant microorganism comprising a foreign gene encoding a variant of a nitrous oxide reductase protein, wherein the variant comprises an amino acid alteration of an amino acid residue corresponding to position L452 of the amino acid sequence of SEQ ID NO:
- 1 and has activity of an enzyme belonging to EC 1.7.2.4.
A composition for reducing a concentration of nitrous oxide in a sample, the composition comprising a recombinant microorganism comprising a foreign gene encoding a variant of a nitrous oxide reductase protein, wherein the variant has an amino acid alteration of an amino acid residue corresponding to position L452 of the amino acid sequence of SEQ ID NO:
- 1 and has activity of an enzyme belonging to EC 1.7.2.4.
A method of reducing a concentration of nitrous oxide in a sample, the method comprising contacting a sample comprising nitrous oxide, with a recombinant microorganism comprising a foreign gene encoding a variant of a nitrous oxide reductase protein or with a variant of a nitrous oxide reductase protein, wherein the variant comprises an amino acid alteration of an amino acid residue corresponding to position L452 of the amino acid sequence of SEQ ID NO:
- 1 and has activity of an enzyme belonging to EC 1.7.2.4.
A variant of nitrous oxide reductase, comprising an amino acid alteration of an amino acid residue corresponding to position L452 of the amino acid sequence of SEQ ID NO:
- 1, the variant having activity of an enzyme belonging to EC 1.7.2.4, wherein the amino acid alteration is a substitution of the amino acid residue corresponding to position L452 with A, C, F, H, K, M, Q, or S.
A polynucleotide encoding a variant of nitrous oxide reductase, wherein the variant comprises an amino acid alteration of an amino acid residue corresponding to position L452 of the amino acid sequence of SEQ ID NO:
- 1 and has activity of an enzyme belonging to EC 1.7.2.4, and wherein the amino acid alteration is a substitution of the amino acid residue corresponding to position L452 with A, C, F, H, K, M, Q, or S.
Dependencies are inferred from the claim text.