Engineered binding proteins for recognition of bacteria
AssigneeMassachusetts Institute of Technology
InventorsHadley Sikes Johnson, Ki-Joo Sung, Yining Hao
Life of this patent
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Abstract
Described herein are antigen-binding molecules that bind to bacteria (e.g., Listeria monocytogenes) and methods of use thereof. Also described herein are compositions and kits comprising these antigen-binding molecules.
Claims119
An antigen-binding molecule comprising a reduced charge Sso7d(rcSso7d) antigen-binding protein that binds to a bacterium.
An antigen-binding molecule comprising a reduced charge Sso7d (rcSso7d) antigen-binding protein that binds Listeria monocytogenes protein LMOf2365_0639, wherein the antigen-binding protein comprises a variable region having:
- (i) the amino acid sequence of any one of SEQ ID NOs:
- 1-8;
- or
- (ii) an amino acid sequence having 2 or 1 amino acid differences with any one of SEQ ID NOs:
- 1-8.
An antigen-binding molecule comprising a reduced charge Sso7d (rcSso7d) antigen-binding protein that binds Salmonella typhi Native Omp, said Native Omp comprising the amino acid sequence of Salmonella typhi membrane proteins OmpA, OmpW, OmpC, OmpF, OmpD, and phoE, wherein the antigen-binding protein comprises a variable region having:
- (i) the amino acid sequence of any one of SEQ ID NOs:
- 58-71;
- or
- (ii) an amino acid sequence having 2 or 1 amino acid differences with any one of SEQ ID NOs:
- 58-71.
An antigen-binding molecule comprising a reduced charge Sso7d (rcSso7d) antigen-binding protein that binds E. coli OmpW, wherein the antigen-binding protein comprises a variable region having:
- (i) the amino acid sequence of any one of SEQ ID NOs:
- 73-80;
- or
- (ii) an amino acid sequence having 2 or 1 amino acid differences with any one of SEQ ID NOs:
- 73-80.
The antigen-binding molecule of claim 33 or claim 34, wherein the rcSso7d antigen-binding protein comprises the amino acid sequence of any one of SEQ ID NOs:
- 47-54.
An antigen-binding molecule comprising a reduced charge Sso7d (rcSso7d) antigen-binding protein that binds E. coli OmpA, wherein the antigen-binding protein comprises a variable region having:
- (i) the amino acid sequence of any one of SEQ ID NOs:
- 81-83;
- or
- (ii) an amino acid sequence having 2 or 1 amino acid differences with any one of SEQ ID NOs:
- 81-83.
Dependencies are inferred from the claim text.