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US 2023/0348969 A1Published application
Methods and systems for nucleic acid sequencing
AssigneeUltima Genomics, Inc.
InventorsMark PRATT, Florian OBERSTRASS, Linda LEE, Steven MENCHEN
Life of this patent
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Abstract
The present disclosure provides methods and systems for processing a nucleotide mixture. A nucleotide mixture can be purified. A nucleotide mixture can be processed for use in nucleic acid synthesis. A nucleotide mixture can be processed for use in nucleic acid sequencing.
Claims21
-125. (canceled)
A method for processing a mixture, comprising:
- (a) selecting from a set of canonical types of nucleotides or nucleotides analogs a subset of canonical types of nucleotides or nucleotide analogs;
- (b) directing a mixture comprising a plurality of nucleotides or nucleotide analogs to a reaction space comprising a support having a plurality of nucleic acid molecules immobilized thereto, wherein a percentage of nucleotides or nucleotide analogs corresponding to said subset relative to all other nucleotides or nucleotide analogs in said mixture is greater than 50%;
- and
- (c) incorporating nucleotides or nucleotide analogs that do not correspond to said subset from said mixture into said plurality of nucleic acid molecules such that said percentage is increased following said incorporating;
- wherein (a)-(c) are performed in absence of sequencing or sequence identification of said plurality of nucleic acid molecules.
2 more elements
A method for processing a mixture, comprising:
- (a) selecting from a set of canonical types of nucleotides or nucleotides analogs a subset of canonical types of nucleotides or nucleotide analogs;
- (b) directing a mixture comprising a plurality of nucleotides or nucleotide analogs to a reaction space comprising a support having a plurality of nucleic acid molecules immobilized thereto, wherein a percentage of nucleotides or nucleotide analogs corresponding to said subset relative to all other nucleotides or nucleotide analogs in said mixture is less than 20%;
- and
- (c) incorporating said nucleotides or nucleotide analogs corresponding to said subset from said mixture into said plurality of nucleic acid molecules such that said percentage is reduced following incorporation,
- wherein (a)-(c) are performed in absence of sequencing or sequence identification of said plurality of nucleic acid molecules.
2 more elements
A method for synthesizing a nucleic acid molecule, comprising:
- (a) directing a plurality of nucleotides or nucleotide analogs to a reaction space comprising a support having a plurality of nucleic acid molecules immobilized thereto;
- (b) incorporating a subset of nucleotides or nucleotide analogs from said plurality of nucleotides or nucleotide analogs into said plurality of nucleic acid molecules, thereby providing a remainder of said plurality of nucleotides or nucleotides analogs;
- and
- (c) using said remainder of said plurality of nucleotides or nucleotide analogs to perform nucleic acid synthesis, thereby synthesizing said nucleic acid molecule;
- wherein (a)-(b) are performed in absence of sequencing or sequence identification of said plurality of nucleic acid molecules.
2 more elements
Dependencies are inferred from the claim text.