Burden-addicted production strains
OwnerPeter RUGBJERG, William Kristian Pallisgaard OLSEN +2inventors
InventorsPeter RUGBJERG, William Kristian Pallisgaard OLSEN, Morten Otto Alexander SOMMER, Anne Line Tidemand JOHANSEN
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Abstract
The invention provides a microbial production cell for synthesis of a product, further comprising a burden-addiction genetic circuit whose expression confers a selective growth and/or survival advantage on those cells that synthesize the product; while limiting proliferation of low- or non-productive escaper cells.
Claims15
A microbial production cell genetically engineered to synthesize a product, said microbial cell further comprising:
- a. an essential gene operably linked to a burden-sensing promoter, wherein said promoter is heterologous with respect to said essential gene;
- wherein synthesis of the product confers a burden on said cell, and wherein expression of said essential gene is up-regulated when said burden-sensing promoter is induced by said burden relative to a basal level expression of said essential gene when said burden-sensing promoter is not induced.
Use of an essential gene operably linked to a burden-sensing promoter to enhance product yield of a cultured population of microbial production cells arising from a single cell,
- wherein said promoter is heterologous with respect to said essential gene, wherein production of the product confers a burden on said cell, and wherein expression of said essential gene is up-regulated when said burden-sensing promoter is induced by said burden relative to a basal level expression of said essential gene when said burden-sensing promoter is not induced.
Dependencies are inferred from the claim text.