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AssigneeUniversity Industry Foundation, Yonsei University Wonju Campus
InventorsYoung Joon SEO, Dong Jun PARK, Jeong Eun PARK
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The present invention relates to a composition for preventing hearing loss, containing mesenchymal stem cells (MSC) or exosomes derived therefrom as an active ingredient. Specifically, an effect of preventing damage to the inner hair cells and outer hair cells of the cochlea caused by ototoxic drugs is exerted since a large amount of HSP70 protein is contained in exosomes contained in an MSC culture solution obtained according to the present invention, MSCs co-cultured with cochlear explants according to the present invention, and exosomes contained in a co-culture solution of MSCs and cochlear explants, and thus the MSCs co-cultured with cochlear explants, culture solution, and exosomes isolated therefrom can be usefully utilized as an active ingredient of a composition for preventing hearing loss.
A method for preventing hearing loss, the method comprising administering a pharmaceutically effective amount of mesenchymal stem cells (MSC) co-cultured with cochlear explants, a co-culture solution of mesenchymal stem cells and cochlear explants exosomes isolated from the co-culture solution, a mesenchymal stem cell culture solution or exosomes isolated from the culture solution to a subject.
-20. (canceled)
A method for preparing mesenchymal stem cells for preventing hearing loss comprising the following step 1) and 2), or preparing mesenchymal stem cells and cochlear explants for preventing hearing loss comprising the following step 1):
The method according to claim 15, wherein the mesenchymal stem cells and the cochlear explants are co-cultured in a state of being spatially separated from each other in a co-culture medium as the mesenchymal stem cells are positioned in a transwell upper chamber and the cochlear explants are positioned in a transwell lower chamber in step 1).
(canceled)
A method for preparing a co-culture solution of mesenchymal stem cells and cochlear explants for preventing hearing loss comprising the following step 1) and 2), or preparing exosomes isolated from the co-culture solution of mesenchymal stem cells and cochlear explants for preventing hearing loss comprising the following step 1) to 3):
The method according to claim 17, wherein the mesenchymal stem cells and the cochlear explants are co-cultured in a state of being spatially separated from each other in a co-culture medium as the mesenchymal stem cells are positioned in a transwell upper chamber and the cochlear explants are positioned in a transwell lower chamber in step 1).
The method according to claim 17, wherein the co-culture in step 1) is performed for 12 hours or more.
-31. (canceled)
Dependencies are inferred from the claim text.