Systems and methods for generation of hyperpolarized compounds using parahydrogen

A method of generating a solution comprising a hyperpolarized target compound, the method comprising:

• generating a first solution including a target compound, parahydrogen, and a catalyst, the catalyst configured to bind the parahydrogen and the target compound;
• transferring spin order from the parahydrogen to polarization on the target compound via the catalyst using the SABRE effect;
• generating a second solution including the target compound and the catalyst, the generation comprising:
• generating a precipitate of the target compound;
• separating the precipitate from the first solution;
• and
• combining at least some of the precipitate with a solvent, thereby generating the solution comprising the hyperpolarized target compound.

A system for generating a polarized solution comprising a hyperpolarized target compound, the system comprising:

• a first sub-system for generating a hyperpolarized precipitate, the first sub-system comprising:
• a hydrogenation device configured to generate a first solution by combining parahydrogen gas, a solvent, and a precursor or a target compound;
• a polarization device configured to receive the first solution and generate a second solution, the polarization device including a polarization chamber having a volume of at least 1 ml;
• a waveform generator coupled to the polarization device, the waveform generator configured to provide a polarization transfer signal;
• and
• a separation system configured to separate a precipitated fraction of the hyperpolarized target compound from the second solution;
• and
• a second sub-system for dissolution of the precipitated fraction, the second sub-system comprising a dissolution chamber configured to contain the precipitated fraction and to receive a second solvent for dissolving the precipitated fraction.

A system, comprising:

• a magnetic shield;
• a sample reservoir disposed within the magnetic shield;
• a magnetic field coil disposed within the magnetic shield, electrically connected to a signal generator, and configured to generate, when driven by the signal generator, a magnetic field, a spatial deviation of the magnetic field being, when an amplitude of the magnetic field is less than 2 μT, less than 0.5 uT over a volume of the sample reservoir, the volume of the sample reservoir being greater than 10 ml and less than 2,000 ml;
• and
• at least one computer-readable media containing instructions that, when executed by at least one processor of the system, cause the system to perform operations comprising:
• modulating the amplitude of the magnetic field, a duration of the modulation being greater than 100 milliseconds and less than 20,000 milliseconds, the amplitude of the magnetic field during the modulation being less than 2 uT;
• and
• wherein the modulation is configured to transfer a population difference in parahydrogenated proton spin states to polarization on a target nuclear spin of a target compound in a sample contained in the sample reservoir.