Bacterial strains for dna production
AssigneeModernaTX, Inc.
InventorsKevin Smith, Marcus Duvall, Bhargav Tilak
Life of this patent
Estimating the term…
Abstract
Compositions for the production of plasmid nucleic acids and methods of making and using the same are provided.
Claims75
An engineered nucleic acid vector comprising a stationary-phase-induced promoter and a primosome assembly site (PAS).
A recombinant plasmid comprising the geneotype:|<repA|ori_ts|<recA|<bla|<tetR|<P(tetR)|P(tet)>|gamma>|beta>|exo>|a>|.
A recombinant plasmid comprising a nucleic acid sequence with at least 70% identity to SEQ ID NO:
- 19.
A recombinant plasmid comprising a nucleic acid sequence with at least 70% identity to SEQ ID NO:
- 20.
A nucleic acid comprising a prsA variant.
A genetically modified microorganism comprising a prsA variant, wherein the microorganism has a genome in which a repressor gene purR has been disrupted.
A recombinant strain of Escherichia coli (E. coli), comprising:
- an E. coli genome with at least the following gene deletions:
- endA (ΔendA) and recA (ΔrecA).
A genetically modified microorganism comprising Strain 3.
A genetically modified microorganism comprising Strain 4.
An engineered nucleic acid vector comprising a nucleic acid having at least 70% sequence identity to SEQ ID NO:
- 21.
An engineered nucleic acid vector comprising a nucleic acid having at least 80% sequence identity to SEQ ID NO:
- 21.
An engineered nucleic acid vector comprising a nucleic acid having at least 90% sequence identity to SEQ ID NO:
- 21.
An engineered nucleic acid vector comprising a nucleic acid having at least 95% sequence identity to SEQ ID NO:
- 21.
An engineered nucleic acid vector comprising a nucleic acid having SEQ ID NO:
- 21.
An engineered nucleic acid vector comprising a nucleic acid having at least 70% sequence identity to SEQ ID NO:
- 22.
An engineered nucleic acid vector comprising a nucleic acid having at least 80% sequence identity to SEQ ID NO:
- 22.
An engineered nucleic acid vector comprising a nucleic acid having at least 90% sequence identity to SEQ ID NO:
- 22.
An engineered nucleic acid vector comprising a nucleic acid having at least 95% sequence identity to SEQ ID NO:
- 22.
An engineered nucleic acid vector comprising a nucleic acid having SEQ ID NO:
- 22.
An engineered nucleic acid vector comprising a nucleic acid sequence having at least 70% sequence identity to any one of SEQ ID NO:
- 1-15.
An engineered nucleic acid vector comprising a nucleic acid sequence having at least 80% sequence identity to any one of SEQ ID NO:
- 1-15.
An engineered nucleic acid vector comprising a nucleic acid sequence having at least 90% sequence identity to any one of SEQ ID NO:
- 1-15.
An engineered nucleic acid vector comprising a nucleic acid sequence having at least 95% sequence identity to any one of SEQ ID NO:
- 1-15.
An engineered nucleic acid vector comprising a nucleic acid sequence having at least 99% sequence identity to any one of SEQ ID NO:
- 1-15.
An engineered nucleic acid vector comprising a nucleic acid sequence of any one of SEQ ID NO:
- 1-15.
An engineered nucleic acid vector comprising a nucleic acid sequence having at least 70% sequence identity to SEQ ID NO:
- 10.
An engineered nucleic acid vector comprising a nucleic acid sequence having at least 70% sequence identity to SEQ ID NO:
- 11.
An engineered nucleic acid vector comprising a nucleic acid sequence having at least 95% sequence identity to SEQ ID NO:
- 10.
An engineered nucleic acid vector comprising a nucleic acid sequence having at least 95% sequence identity to SEQ ID NO:
- 11.
An engineered nucleic acid vector comprising a nucleic acid sequence having at least 99% sequence identity to SEQ ID NO:
- 10.
An engineered nucleic acid vector comprising a nucleic acid sequence having at least 99% sequence identity to SEQ ID NO:
- 11.
An engineered nucleic acid vector comprising a nucleic acid sequence of SEQ ID NO:
- 10.
An engineered nucleic acid vector comprising a nucleic acid sequence of SEQ ID NO:
- 11.
Dependencies are inferred from the claim text.