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US 2023/0279233 A1Published application
Atrorosins as food colors
AssigneeCHROMOLOGICS ApS
InventorsHenrik Dalbøge, Anders Sebastian Rosenkrans Ødum
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Abstract
The present invention provide scalable methods of producing newly identified atrorosin pigments, compositions comprising the pigments, lakes and dyes and uses thereof for colorings foods, such as dairy, meat substitutes or candy.
Claims33
A method for preparing an Atrorosin food coloring composition from a fermentation broth, comprising:
- a. Removing the biomass and other macro-sized constituents by membrane filtration;
- b. Removing proteins, peptides and other constituents by ultrafiltration through an ultrafiltration membrane with a cutoff between 1 kDa to 100 kDa;
- c. Performing acid precipitation of the Atrorosin of the permeate of step b, wherein the pH is lowered to be below the pKa of the Atrorosin;
- d. Filtering the precipitate of step c by membrane filtration (with a membrane having a pore size below 1 μm;
- e. Adding a buffer to the precipitate of step d to increase the pH to above the pKa of the Atrorosin of the precipitate;
- and
- f. Drying the mixture of step e to remove water to make an Atrorosin powder comprising a salt composition of Atrorosin and salt of the buffer.
4 more elements
2-32. (canceled)
A composition comprising Atrorosin, wherein at least 80% of the Atrorosin is cis Atrorosin, and less than 20% is trans Atrorosin.
Dependencies are inferred from the claim text.