Method and illumination device of the adaptive optics in transmission or reflection microscopy

12:

• A method for optimizing parameters of a physical light propagation model, the method comprising:
• providing a physical model Mlight of a light propagation in an optical system;
• radiating an input light distribution I0 using an illumination unit into an excitation path of the optical system;
• traversing the input light distribution I0 through a scattering body, wherein the scattering body is arranged in the excitation path of the optical system and modifies the input light distribution I0 to form a transmission light distribution ITR or I0 to form a reflection light distribution IRE;
• recording the transmission light distribution ITR or the reflection light distribution IRE;
• transferring the recorded transmission light distribution ITR or the recorded reflection light distribution IRE to the physical model Mlight;
• and
• computing transmission distortion parameters VTR of the physical model Mlight based on the recorded transmission light distribution ITR or the recorded reflection light distribution IRE, wherein the transmission distortion parameters VTR characterize the scattering body.

19:

• The method as recited in claim 12, further comprising:
• reflecting the transmission light distribution ITR at a location of the sample using a reflective material so that the transmission light distribution ITR again traverses the scattering body and is modified to form a reflection light distribution IRE;
• and
• recording the refection light distribution IRE.

22:

• An irradiation device for performing the method as recited in claim 12, the irradiation device comprising:
• a microscope comprising,
• an illumination unit which is designed to generate a light distribution I0, and
• an excitation path comprising a scattering body which is arranged in the excitation path, the excitation path being configured to guide the light distribution I0 to a location of a sample;
• a camera which is configured to record a transmitted light ITR;
• and
• a computer unit comprising an algorithm implemented thereon,
• wherein,
• a physical model Mlight of a light propagation in the irradiation device is provided on the computer unit, and
• the algorithm implemented on the computer unit is configured to compute transmission distortion parameters VTR of the physical model Mlight based on the physical model Mlight and the transmitted light ITR.

24:

• A method for correcting distortions of a light distribution during passage through an optical system which comprises a scattering body, the method comprising:
• extracting a transmission distortion parameter VTR as computed in claim 12;
• and
• setting a complementary distortion pattern VTR# on an optical modulator of an excitation path,
• wherein,
• the optical modulator is arranged in a beam direction before the scattering body.

27:

• An irradiation device for performing the method as recited in claim 24, the irradiation device comprising:
• a microscope comprising,
• an illumination unit which is configured to generate a light distribution I0, and
• an excitation path comprising a scattering body which is arranged in the excitation path, the excitation path being configured to guide the light distribution I0 to a location of a sample;
• an optical modulator arranged in the excitation path in a light propagation direction before the scattering body;
• and
• a computer unit which is configured to,
• control the optical modulator,
• to extract transmission distortion parameters VTR from the physical model Mlight of the light propagation in the irradiation device, and
• to set the optical modulator using a complementary distortion pattern VTR# correcting distortions of the scattering body.