US 2023/0273232 A1 · ROBOTIC MICROINJECTION

Abstract

Apparatus and methods for more efficiently micro-positioning a micropipette with respect to an egg and/or injecting fluid into a plurality of eggs using a micropipette are provided. Various approaches described herein comprise use of automated and/or robotic systems to direct a micropipette to and inject the micropipette in eggs mounted on a sample holder.

Claims37

Dependent claims

A micro-positioning system configured to insert a pipette into a plurality of eggs, said micro-positioning system comprising:

a pipette holder configured to secure said pipette to said pipette holder;
a shaft for mounting a plurality of eggs, said pipette holder movable in lateral, longitudinal, and vertical directions with respect to said shaft to position said pipette with respect to said eggs;
at least one electronically controlled lateral translation stage configured to translate in the lateral direction so as to move said pipette holder laterally with respect to said shaft;
at least one electronically controlled longitudinal translation stage configured to translate in the longitudinal direction so as to move said pipette holder longitudinally with respect to said shaft;
at least one vertical translation stage configured to translate in the vertical direction so as to move said pipette holder vertically with respect to said shaft;
a motor connected to said shaft to rotate said shaft so as to provide said pipette access to different eggs mounted on said shaft;
and
electronics configured to drive at least said lateral and longitudinal translation stages and said motor to position said pipette with respect to said egg.

5 more elements

Dependencies are inferred from the claim text.

Facts

Application

17/976,677Filed 28 Oct 2022

Priority

25 Feb 2022

Published

31 Aug 2023

Term ends

Not estimatedThe source records don’t support an estimate.

Classification10 codes

B25J 7/00
Micromanipulators
G01N 35/1011
Characterised by arrangements for controlling the aspiration or dispense of liquids: control of the position or alignment of the transfer device
G01N 23/2204
By measuring secondary emission from the material: specimen supports therefor; Sample conveying means therefore
G01N 35/00732
Communications; Identification: identification of carriers, materials or components in automatic analysers

Abstract

Claims37

Dependent claims

A micro-positioning system configured to insert a pipette into a plurality of eggs, said micro-positioning system comprising:

a pipette holder configured to secure said pipette to said pipette holder;
a shaft for mounting a plurality of eggs, said pipette holder movable in lateral, longitudinal, and vertical directions with respect to said shaft to position said pipette with respect to said eggs;
at least one electronically controlled lateral translation stage configured to translate in the lateral direction so as to move said pipette holder laterally with respect to said shaft;
at least one electronically controlled longitudinal translation stage configured to translate in the longitudinal direction so as to move said pipette holder longitudinally with respect to said shaft;
at least one vertical translation stage configured to translate in the vertical direction so as to move said pipette holder vertically with respect to said shaft;
a motor connected to said shaft to rotate said shaft so as to provide said pipette access to different eggs mounted on said shaft;
and
electronics configured to drive at least said lateral and longitudinal translation stages and said motor to position said pipette with respect to said egg.

5 more elements

Dependencies are inferred from the claim text.

Robotic microinjection

Life of this patent

Abstract

Claims37

Robotic microinjection

Life of this patent

Abstract

Claims37