Methods and Compositions for Diagnosing and Treating Cancer
OwnerCatherine Lynn Hedrick, Melissa Meyerinventors
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Abstract
The present invention includes methods for detecting B-cell like neutrophils (“BNeuts”) comprising: obtaining a biological sample from a subject; and detecting whether BNeuts are present or increased in the biological sample by contacting the biological sample with an agent capable of detecting CD3− CD56−CD79b+CD66b+ PAX5+CD19− cells, CD3−CD56−CD79a+CD79b+CD66b+ PAX5+CD19− cells, or both, and detecting the increase of BNeuts in the biological sample.
Claims33
A method of detecting B-cell like neutrophils (“BNeuts”) comprising:
- obtaining a biological sample from a subject;
- and
- detecting whether BNeuts are present or increased in the biological sample by contacting the biological sample with one or more agents capable of detecting CD3−CD56−CD79b+CD66b+PAX5+CD19− cells, CD3− CD56−CD79a+CD79b+CD66b+PAX5+CD19− cells, or both.
A method of diagnosing and treating a cancer in a patient, the method comprising the steps of:
- determining whether the patient has, or has an increase of, B-cell like neutrophils (BNeut) cells by:
- obtaining or having obtained a biological sample from the patient;
- performing or having performed an assay on the biological sample to determine an amount of BNeuts in the biological sample, wherein the BNeuts are CD3−CD56−CD79b+CD66b+PAX5+CD19− cells, CD3−CD56−CD79a+CD79b+CD66b+PAX5+CD19− cells, or both;
- identifying that the patient has a presence or an increase in BNeuts when compared to a reference level generated from a healthy patient;
- and
- if the patient has BNeuts, or shows an increase in BNeuts, administering a cancer treatment or therapy to the patient.
A method for treating a patient suffering from a cancer, the method comprising the steps of:
- determining whether the patient has B-cell like neutrophils (BNeut) or an increase in BNeuts, when compared to a reference level generated for a healthy patient by:
- obtaining or having obtained a biological sample from the patient;
- and
- performing or having performed an assay on the biological sample to determine if the patient has BNeuts;
- and
- if the patient has BNeuts, then administering a cancer treatment or therapy to the patient.
A method for treating a cancer patient, the method comprising the steps of:
- determining whether the cancer patient has an increase in B-cell like neutrophils (“BNeuts”) by:
- obtaining or having obtained a biological sample from the patient;
- performing or having performed an assay on the biological sample to determine if the patient has an increase in BNeuts when compared to a baseline level of BNeuts present in a healthy patient;
- and
- if the patient has an increase in BNeuts when compared to a baseline level of BNeuts present in the healthy patient, then providing an adoptive cell therapy that comprises BNeuts, CD3−CD56−CD79b+CD66b+PAX5+CD19− cells, CD3−CD56−CD79a+CD79b+CD66b+PAX5+CD19− cells, or any combination thereof, to the patient in an amount sufficient to reduce or treat the cancer.
A kit for cell sorting or detecting B-cell like neutrophils (“BNeuts”) from a biological sample, the kit comprising detecting agents for at least one of:
- CD3−CD56−CD79b+CD66b+PAX5+CD19− cells, CD3− CD56−CD79a+CD79b+CD66b+PAX5+CD19− cells, or both.
A method for treating a patient, the method comprising the steps of:
- identifying that the patient is in need of a treatment for cancer;
- and
- providing an adoptive cell therapy that comprises BNeuts, CD3−CD56−CD79b+CD66b+PAX5+CD19− cells, CD3−CD56−CD79a+CD79b+CD66b+PAX5+CD19− cells, or any combination thereof, to the patient in an amount sufficient to reduce or treat the cancer, wherein the BNeuts are autologous, syngeneic, allogeneic, or xenogeneic.
Dependencies are inferred from the claim text.