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AssigneePioneer Hi-Bred International, Inc.
InventorsALBERT L. LU, MARK EDWARD NELSON, GUSUI WU, TAKASHI YAMAMOTO
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The disclosure provides nucleic acids, and variants and fragments thereof, derived from strains of Bacillus thuringiensis encoding variant polypeptides having increased pesticidal activity against insect pests, including Lepidoptera and Coleopteran. Particular embodiments of the disclosure provide isolated nucleic acids encoding pesticidal proteins, pesticidal compositions, DNA constructs, and transformed microorganisms and plants comprising a nucleic acid of the embodiments. These compositions find use in methods for controlling pests, especially plant pests.
A DNA construct comprising a polynucleotide encoding a one Cry1B variant polypeptide with a second polynucleotide encoding a different second Cry1B variant polypeptide, wherein the first Cry1B variant polypeptide and the second Cry1B variant polypeptide, wherein the first and second Cry1B variant polypeptides each have insecticidal activity.
A transgenic plant comprising a molecular stack comprising a polynucleotide encoding a one Cry1B variant polypeptide with a second polynucleotide encoding a different second Cry1B variant polypeptide, wherein the first Cry1B variant polypeptide and the second Cry1B variant polypeptide, wherein the first and second Cry1B variant polypeptides each have insecticidal activity.
A transgenic plant comprising a breeding stack of a polynucleotide encoding a one Cry1B variant polypeptide with a second polynucleotide encoding a different second Cry1B variant polypeptide, wherein the first Cry1B variant polypeptide and the second Cry1B variant polypeptide, wherein the first and second Cry1B variant polypeptides each have insecticidal activity.
A composition comprising a polynucleotide encoding a one Cry1B variant polypeptide with a second polynucleotide encoding a different second Cry1B variant polypeptide, wherein the first Cry1B variant polypeptide and the second Cry1B variant polypeptide, wherein the first and second Cry1B variant polypeptides each have insecticidal activity.
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