Solid support for synthesizing nucleic acid sequences and methods for making and using
AssigneeThe United States of America, as represented by the Secretary, Department of Health and Human Services
InventorsSerge L. Beaucage, Andrzej M. Grajkowski
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Abstract
Disclosed herein are embodiments of a solid support suitable for synthesizing nucleic acid sequences. The solid support may have a structure according to Formula I, where CPG is controlled pore glass, and m, n, x, y, R1 and R2 are as defined herein. Also disclosed are methods for making and using the solid support, kits including solid support, and a universal linker phosphoramidite suitable for use in the solid support.
Claims46
A solid support according to Formula I
- wherein:
- CPG is controlled pore glass;
- m is 2, 3, 4, 5 or 6;
- x is 1, 2, 3, 4 or 5;
- y is from 2 to 12;
- n is 3, 4, 5, 6, 7, 8, 9 or 10;
- each R1 independently is C1-6alkyl, —(CH2)1-6CN, —(CH2)1-6OR′ or a thermolytic phosphate protecting group;
- R′ is aliphatic, aryl, or aralkyl;
- R2 is H,
- p is 2, 3, 4, 5, 6, 7, 8, 9 or 10;
- R3 is H or
- R4 is H or OR6;
- R5 is PG or a nucleic acid sequence;
- R6 is pixyl, TBDMS, TBDPS, TMS, TES, or TIPS.
- T is 1, 2, 3, or 4;
- BP is a nucleic acid base where an exocyclic amine, if present, is protected;
- and
- PG is a protecting group.
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Dependencies are inferred from the claim text.