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US 2023/0270833 A1Published application
Modulation of replicative fitness by deoptimization of synonymous codons
AssigneeThe Government of the U.S.A. as represented by the Secretary of the Dept. of Health & Human Services
InventorsOlen M. Kew, Cara Carthel Burns, Jing Shaw, Raymond Campagnoli, Jacqueline Quay
Life of this patent
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Abstract
Methods of producing a pathogen with reduced replicative fitness are disclosed, as are attenuated pathogens produced using the methods. In particular examples, the method includes deoptimizing one or more codons in a coding sequence, thereby reducing the replicative fitness of the pathogen. Methods of using the attenuated pathogens as immunogenic compositions are also disclosed.
Claims13
1-49. (canceled)
A modified dengue virus, comprising:
- a surface glycoprotein coding sequence of the dengue virus, wherein at least eight codons in the coding sequence are deoptimized as compared to its native coding sequence,
- wherein the at least eight deoptimized codons are each a synonymous codon less frequently used in the native dengue virus, wherein the synonymous codon less frequently used in the native dengue virus is a codon that encodes the same amino acid, but the codon is an unpreferred codon by the native dengue virus for the amino acid.
Dependencies are inferred from the claim text.