Method for diagnosis based on circulating extracellular vesicles
AssigneeWellsim Biomedical Technologies, Inc
InventorsYuchao CHEN, Katherine TUCHEZ
Life of this patent
Estimating the term…
Abstract
The present disclosure provides a method for diagnosis based on proteins present on the surface of circulating extracellular vesicles. The method comprises incubating a sample of the subject with a detection antibody linked to a detectable label, contacting the sample with a capture antibody immobilized on a substrate, and detecting the detectable label on the circulating EV immobilized on the substrate. Compared to the method currently known in the art, the method disclosed herein has the advantages of high sensitivity with low cost and rapid procedure, high specificity.
Claims22
A method for preparing a circulating extracellular vesicle (EV) from a subject useful for analyzing Alzheimer's disease (AD) in the subject, the method comprising:
- incubating a sample of the subject with a detection antibody linked to a detectable label, wherein the sample comprises a circulating EV derived from neurons, wherein the detection antibody specifically binds to an AD-specific antigen present on the surface of the circulating EV derived from neurons;
- contacting the sample with an anti-CD56 antibody immobilized on a substrate, thus immobilizing the circulating EV derived from neurons on the substrate;
- and
- detecting the detectable label on the circulating EV immobilized on the substrate.
2-4. (canceled)
(canceled)
A method for preparing a circulating EV from a subject useful for analyzing AD in the subject, the method comprising:
- incubating a sample of the subject with a first detection antibody linked to a first detectable label and a second detection antibody linked to a second detectable label, wherein the sample comprises a circulating EV derived from neurons, wherein the first detection antibody and the second detection antibody specifically bind to a first AD-specific antigen and a second AD-specific antigen present on the surface of the circulating EV derived from neurons respectively;
- contacting the sample with an anti-CD56 antibody immobilized on a substrate, thus immobilizing the circulating EV derived from neurons on the substrate;
- and
- detecting the first detectable label and the second detectable label on the circulating EV immobilized on the substrate.
(canceled)
Dependencies are inferred from the claim text.