Protein microarray, use and detection method thereof

A protein microarray, comprising:

• a substrate comprising a plurality of protein array blocks on a surface of the substrate;
• and
• at least two proteins immobilized on each of the plurality of protein array blocks, wherein the at least two proteins comprise an extracellular domain or a receptor binding domain of a spike protein from a virus, and an extracellular region or a receptor binding domain of a spike protein from a variant of the virus,
• wherein the extracellular domain of the spike protein of the virus comprises an amino acid sequence of SEQ ID NO:
• 1;
• the receptor binding domain of the spike protein of the virus comprises an amino acid sequence of SEQ ID NO:
• 10;
• the extracellular domain of the spike protein of the variant of the virus comprises any one of an amino acid sequence of SEQ ID NO:
• 2, SEQ ID NO:
• 3, SEQ ID NO:
• 4, SEQ ID NO:
• 5, SEQ ID NO:
• 6, SEQ ID NO:
• 7, SEQ ID NO:
• 8, SEQ ID NO:
• 9, or any combination thereof;
• and
• the receptor binding domain of the spike protein of the variant of the virus comprises any one of an amino acid sequence of SEQ ID NO:
• 11, SEQ ID NO:
• 12, SEQ ID NO:
• 13, SEQ ID NO:
• 14, SEQ ID NO:
• 15, SEQ ID NO:
• 16, SEQ ID NO:
• 17, or any combination thereof.