Enhanced chemiluminescent enzyme-linked immunosorbent assay for detection of antibodies against babesia microti
AssigneeThe U.S.A., as represented by the Secretary, Department of Health and Human Services
InventorsSanjai Kumar, Nitin Verma, Ankit Puri
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Abstract
Identification of immunodominant Babesia microti antigens using genome-wide immunoscreening is described. Candidate antigens were screened against sera from patients with clinical babesiosis. Also described are diagnostic assays with high sensitivity and specificity for detecting B. microti-specific antibodies in patient samples using the identified immunodominant antigens.
Claims18
An isolated complementary DNA (cDNA) encoding a Babesia microti antigenic polypeptide, wherein the amino acid sequence of the B. microti antigenic polypeptide consists of SEQ ID NO:
- 2 or SEQ ID NO:
- 4.
A vector comprising a complementary DNA (cDNA) encoding a Babesia microti antigenic polypeptide operably linked to a heterologous promoter, wherein the amino acid sequence of the B. microti antigenic polypeptide is at least 90% identical to SEQ ID NO:
- 2 or SEQ ID NO:
- 4.
Dependencies are inferred from the claim text.