Color-based and/or visual methods for identifying the presence of a transgene and compositions and constructs relating to the same
AssigneePairwise Plants Services, Inc.
InventorsNicholas Bate, Shai Joshua Lawit
Life of this patent
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Abstract
Described herein are color-based and/or visual methods for identifying the presence of a transgene (e.g., the presence of a transgene in a cell, seed, plant part, and/or plant) along with composition, systems, and constructs relating to the same.
Claims34
A method of identifying a seed and/or plant that is devoid of a transgene, the method comprising:
- providing a plurality of seeds, wherein the plurality of seeds comprises a first seed that is devoid of the transgene and has a first color;
- visually inspecting the plurality of seeds;
- and
- identifying one or more seed(s) from the plurality of seeds that have the first color, thereby identifying the seed and/or plant that is devoid of the transgene.
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The method of claim 18, wherein the promoter comprises a nucleotide sequence having at least 70%, 75%, 80%, 85%, 90%, 95%, 96%, 97%, 98%, 99%, or more sequence identity to SEQ ID NO:82 or 83.
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An expression cassette comprising a first nucleic acid encoding a color conferring polypeptide and a second nucleic acid encoding and/or comprising all or a portion of an editing system.
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A method of identifying a seed that includes a transgene, the method comprising:
- providing a plurality of seeds, wherein the plurality of seeds comprises a first seed having a first color and/or a second seed having a second color, wherein the second color indicates the presence of the transgene, and the first color and second color are different;
- visually inspecting the plurality of seeds;
- and
- identifying one or more seed(s) from the plurality of seeds that have the second color, thereby identifying the seed that includes the transgene.
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Dependencies are inferred from the claim text.