US 2023/0266285 A1 · LOW TEMPERATURE OLIGOSACCHARIDE ANALYTICAL SYSTEM AND METHOD

Abstract

A novel analytical system and method to analyze complex carbohydrates such as human milk oligosaccharides by low-temperature High-Performance Anion Exchange Chromatography with Pulsed Amperometric Detection and High-resolution Mass Spectrometry (HPAE-PAD-MS). The analytical system controls the temperature of the column, electrochemical detector, and ion removal device at or below 15° C. The HPAE-PAD workflow with high-resolution mass spectrometry provides useful molecular structure information. It facilitates the detection of milk oligosaccharides, particularly unknown structures, without the use of analytical standards.

Claims19

Dependent claims

An analytical system comprising:

a) a pump configured to pump a mobile phase;
b) an injection valve configured to input a sample into the mobile phase, the injection valve is fluidly coupled to an output of the pump;
c) a chromatography column configured to separate a sample into one or more analytes;
d) an electrochemical detector, the electrochemical detector fluidly coupled to an output of the chromatography column;
e) an ion removal device configured to remove at least ions of one charge from the mobile phase, the ion removal device fluidly coupled to an output of the electrochemical detector;
and
f) a cooling means for keeping the chromatography column, electrochemical detector, and the ion removal device at a temperature of 15° C. or less.

4 more elements

Dependencies are inferred from the claim text.

Low temperature oligosaccharide analytical system and method

Life of this patent

Low temperature oligosaccharide analytical system and method

Life of this patent

Abstract

Claims19