High throughput liquid chromotography using low flowrate

A method of using a high-duty-cycle liquid chromatography system, comprising:

• obtaining a sample;
• moving a valve to couple a mobile phase gradient delivery pump and a gradient storage chamber;
• pumping, by the mobile phase gradient delivery pump, a mobile phase gradient and the sample to the gradient storage chamber via the valve;
• pumping, by an isocratic pump, a mobile phase A solvent through a column to a waste area;
• once the mobile phase gradient and the sample are entirely pumped into the gradient storage chamber, moving the valve to couple the column with the gradient storage chamber;
• applying a voltage to the column configured to couple the column to a detector;
• pushing the mobile phase gradient and the sample through the column by pumping, by the isocratic pump, the mobile phase A solvent through the column to the detector;
• and
• analyzing the sample by the detector.

A high-duty-cycle liquid chromatography computing system having a processor, a memory, and a storage having stored thereon computer-executable instructions that are structured such that, when the computer-executable instructions are executed by the processor, cause the liquid chromatography system to perform the following:

• identify a column to undergo a regeneration phase;
• move a valve to couple a gradient storage chamber with a mobile phase gradient delivery pump;
• identify that the gradient storage chamber contains a sample and a mobile phase gradient;
• move the valve to couple the gradient storage chamber with the column;
• and
• identify the sample and the mobile phase gradient have moved from the gradient storage chamber to the column.