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US 2023/0266267 A1Published application
SARS-CoV-2 BIOSENSOR UTILIZING A PHOSPHATASE REPORTER
AssigneeUniversity of Southern California
InventorsMoh Amer, Mingrui Chen, Chongwu Zhou
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Abstract
A biosensor capable of detecting both the SARS-CoV-2 S1 spike protein antigen and the SARS-CoV-2 spike protein IgG antibody is disclosed. In various embodiments, the biosensor is configured with an array of solution-gated nanoribbon FETs, wherein each nanoribbon comprises indium oxide (In2O3). In various aspects, the biosensor is fabricated using a scalable and cost-efficient lithography-free process comprising shadow masking.
Claims18
A method of detecting the presence of, or a concentration of, an antigen in a sample, the method comprising:
- fluidically contacting the sample with an array of In2O3 FET devices of a biosensor, the array of In2O3 FET devices configured on a substrate and fluidically accessible by a fluidic cell containing a liquid medium enclosing the biosensor, each In2O3 FET device comprising a source electrode disposed on the substrate, a drain electrode disposed on the substrate, spaced apart from the source electrode, and a In2O3 nanoribbon disposed on the substrate and electrically connecting the source electrode and the drain electrode, wherein each In2O3 nanoribbon in the array of In2O3 FET devices is defined by a channel width, channel length and channel thickness, wherein each In2O3 nanoribbon in the array of In2O3 FET devices includes at least one capture antibody immobilized thereon, the capture antibody being specific to the antigen, and wherein the antigen binds to the immobilized capture antibody;
- fluidically contacting biotinylated secondary antibodies with the array of In2O3 FET devices of the biosensor, the biotinylated secondary antibodies being specific to the antigen, wherein the biotinylated secondary antibody binds to the antigen previously bound to the immobilized capture antibody;
- fluidically contacting streptavidin alkaline phosphatase conjugate with the array of In2O3 FET devices of the biosensor, wherein the alkaline phosphatase binds to the biotinylated secondary antibody through a streptavidin-biotin conjugation;
- fluidically contacting alkaline phosphatase substrate with the array of In2O3 FET devices of the biosensor, the alkaline phosphatase substrate being capable of enzymatic cleavage by the alkaline phosphatase, wherein the alkaline phosphatase cleaves the alkaline phosphatase substrate, producing protons and a pH change in the liquid medium;
- and
- detecting an amperometric signal from the biosensor as a result of the pH change in the liquid medium, indicating a presence of the antigen in the sample.
3 more elements
A method of detecting the presence of, or a concentration of, an antibody in a sample specific to an antigen, the method comprising:
- fluidically contacting the sample with an array of In2O3 FET devices of a biosensor, the array of In2O3 FET devices configured on a substrate and fluidically accessible by a fluidic cell containing a liquid medium enclosing the biosensor, each In2O3 FET device comprising a source electrode disposed on the substrate, a drain electrode disposed on the substrate, spaced apart from the source electrode, and a In2O3 nanoribbon disposed on the substrate and electrically connecting the source electrode and the drain electrode, wherein each In2O3 nanoribbon in the array of In2O3 FET devices is defined by a channel width, channel length and channel thickness, wherein each In2O3 nanoribbon in the array of In2O3 FET devices includes at least one capture antibody immobilized thereon, the capture antibody being specific to and having the antigen bound thereto, and wherein the antibodies in the sample bind to the antigens bound to the immobilized capture antibodies;
- fluidically contacting biotinylated secondary antibodies with the array of In2O3 FET devices of the biosensor, the biotinylated secondary antibodies comprising anti-human antibody to the antigen, wherein the biotinylated secondary antibodies bind to the antibodies previously bound to the antigens immobilized on the capture antibodies;
- fluidically contacting streptavidin alkaline phosphatase conjugate with the array of In2O3 FET devices of the biosensor, wherein the alkaline phosphatase binds to the biotinylated secondary antibody through a streptavidin-biotin conjugation;
- fluidically contacting alkaline phosphatase substrate with the array of In2O3 FET devices of the biosensor, the alkaline phosphatase substrate capable of enzymatic cleavage by the alkaline phosphatase, wherein the alkaline phosphatase cleaves the alkaline phosphatase substrate, producing protons and a pH change in the liquid medium;
- and
- detecting an amperometric signal from the biosensor as a result of the pH change in the liquid medium, indicating a presence of the antibodies in the sample.
3 more elements
Dependencies are inferred from the claim text.